Bacteriology, Department of Infectious and Parasitic Diseases, FARAH and Faculty of Veterinary Medicine, University of Liège, Liège, Belgium.
CEREA, École des Ponts, EDF R&D, IPSL, Marne-la-Vallée, France.
Res Vet Sci. 2022 Dec 20;152:647-650. doi: 10.1016/j.rvsc.2022.09.037. Epub 2022 Oct 4.
Escherichia coli producing Extended-Spectrum-β-Lactamases (ESBL) are a major public health hazard worldwide. The most frequent ESBL belong to the CTX-M family. This study follows their prevalence in pathogenic and non-pathogenic ESBL-producing E. coli isolated from young diarrheic and septicaemic calves over three calving seasons. The triplex PCR targeted three main groups: CTX-M-1, CTX-M-2 and CTX-M-9. Of the 394 isolates studied, 388 (98.5%) were positive, with a majority of CTX-M-1 (243, 61.7%), following by CTX-M-9 (74, 18.8%) and CTX-M-2 (64, 16.2%). The progressive decrease of ESBL-resistance of pathogenic E. coli is not linked to any shift in genetic background, bla genes still present in 99% of the isolates, or to the proportion of the three CTX-M groups. Moreover, no significant difference was observed in the CTX-M content between pathogenic and non-pathogenic E. coli.
产超广谱β-内酰胺酶（ESBL）的大肠杆菌是全球主要的公共卫生危害。最常见的 ESBL 属于 CTX-M 家族。本研究追踪了三个产犊季节中从患有腹泻和败血症的年轻犊牛中分离出的致病性和非致病性产 ESBL 大肠杆菌中 CTX-M-1、CTX-M-2 和 CTX-M-9 这三个主要组的流行情况。三重 PCR 针对三个主要组：CTX-M-1、CTX-M-2 和 CTX-M-9。在所研究的 394 株分离物中，388 株（98.5%）为阳性，其中 CTX-M-1 占大多数（243 株，61.7%），其次是 CTX-M-9（74 株，18.8%）和 CTX-M-2（64 株，16.2%）。致病性大肠杆菌对 ESBL 耐药性的逐渐降低与遗传背景的任何变化、bla 基因仍存在于 99%的分离物中或三种 CTX-M 组的比例无关。此外，在致病性和非致病性大肠杆菌之间，CTX-M 含量没有观察到显著差异。
