首次从印度海洋虾中分离到携带 pESI 巨型质粒的多重耐药性肠炎沙门氏菌血清型婴儿。
First report of a multidrug-resistant Salmonella enterica Serovar Infantis carrying pESI megaplasmid isolated from marine shrimp in India.
机构信息
Quality Control Laboratory, Post-Harvest Technology Department, ICAR-Central Institute of Fisheries Education, Versova, Mumbai, 400061, India.
Quality Control Laboratory, Post-Harvest Technology Department, ICAR-Central Institute of Fisheries Education, Versova, Mumbai, 400061, India.
出版信息
J Glob Antimicrob Resist. 2022 Dec;31:248-251. doi: 10.1016/j.jgar.2022.09.014. Epub 2022 Oct 8.
BACKGROUND
Non-typhoidal Salmonella enterica (NTS) in seafood is an important human health concern. An emerging strain of NTS serovar Infantis carrying a megaplasmid pESI and resistant to multipe drugs has been responsible for frequent food-borne human infections worldwide.
METHODS
S. enterica strain JS5 was isolated from a sample of shrimp from the retail market on XLD agar after enrichment in the RV medium. The genomic DNA was isolated and sequenced using the Illumina platform. The draft whole genome sequence of Salmonella Infantis JS5 revealed the presence of a plasmid.
RESULTS
The genome size was 4,977,731 bp with 4663 open reading frames. The bacterium harboured a megaplasmid similar to the pESI plasmid reported in the emerging S. Infantis. The 285 kb plasmid contained the characteristic genes of the pESI plasmids, such as the mercury operon, yersiniabactin siderophore operon, fimbriae, toxin-antitoxin systems and the hypothetical protein backbone. The antibiotic resistance genes tet(A), dfrA14, aadA, qacEdelta1, and sul1 were detected. To the best of our knowledge, this is the first report on pESI plasmid carrying S. Infantis from India.
背景
海产中的非伤寒型沙门氏菌（NTS）是一个重要的人类健康关注点。一种携带大质粒 pESI 且对多种药物具有耐药性的新型 NTS 肠炎亚种已成为全球频繁发生的食源性人类感染的罪魁祸首。
方法
从零售市场的虾样中，在 RV 培养基中进行富集后，在 XLD 琼脂上分离出 S. enterica 菌株 JS5。使用 Illumina 平台分离并测序基因组 DNA。肠炎沙门氏菌 JS5 的全基因组草图揭示了质粒的存在。
结果
基因组大小为 4977731bp，包含 4663 个开放阅读框。该细菌携带类似于新兴肠炎沙门氏菌中报道的 pESI 质粒的大质粒。285kb 的质粒包含 pESI 质粒的特征基因，如汞操纵子、耶尔森菌铁载体操纵子、菌毛、毒素-抗毒素系统和假设蛋白骨架。检测到抗生素耐药基因 tet(A)、dfrA14、aadA、qacEdelta1 和 sul1。据我们所知，这是首次从印度报告携带 pESI 质粒的肠炎沙门氏菌。
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