Zhang Ruiting, Shen Chao, Zhu De, Le Yu, Wang Nian, Li Yuanxue, Zhang Xianlong, Lin Zhongxu
National Key Laboratory of Crop Genetic Improvement, College of Plant Science & Technology, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
College of Biological and Food Engineering, Guangdong University of Petrochemical Technology, Maoming, 525000, Guangdong, China.
Theor Appl Genet. 2022 Dec;135(12):4483-4494. doi: 10.1007/s00122-022-04233-6. Epub 2022 Oct 11.
A fiber length QTL, qFL-c10-1, was fine-mapped to a 96.5-kb region containing one gene that has not been characterized in plants. Fiber length is an important component of cotton fiber quality, which is associated with other quality properties such as fiber strength, fiber maturity, and fineness. In our previous studies, a stable QTL qFL-c10-1 controlling fiber length had been identified on chromosome A10 in an upland cotton recombinant inbred line (RIL) population from a cross between Jimian5 and DH962. To fine-map qFL-c10-1, an F population with 1081 individual plants from a cross between a recombinant line DJ61 and Jimian5 was established. Using linkage analysis and progeny recombination experiment, qFL-c10-1 was mapped into a 96.5-kb genomic region that just contained one proper transcript Ghir_A10G022020 (described as GhFL10), an undescribed gene in plants. One 214-bp deletion was identified in the promoter region of DJ61 compared with Jimian5. Quantitative real-time PCR (qRT-PCR) and comparative analysis of parental sequences suggested that GhFL10 was the most promising candidate gene for qFL-c10-1. According to RNA-seq, yeast two-hybrid assay and bimolecular fluorescence complementation (BiFC), we speculate that GhFL10 interacts with NF-YA transcription factors to negatively regulate fiber elongation.
一个纤维长度数量性状基因座qFL-c10-1被精细定位到一个96.5千碱基的区域，该区域包含一个在植物中尚未被鉴定的基因。纤维长度是棉花纤维品质的一个重要组成部分，它与其他品质特性如纤维强度、纤维成熟度和细度相关。在我们之前的研究中，在陆地棉重组自交系（RIL）群体（由冀棉5号和DH962杂交产生）的A10染色体上鉴定出一个控制纤维长度的稳定数量性状基因座qFL-c10-1。为了精细定位qFL-c10-1，构建了一个由重组系DJ61和冀棉5号杂交产生的包含1081个单株的F群体。通过连锁分析和后代重组实验，qFL-c10-1被定位到一个96.5千碱基的基因组区域，该区域仅包含一个合适的转录本Ghir_A10G022020（命名为GhFL10），这是一个在植物中未被描述的基因。与冀棉5号相比，在DJ61的启动子区域鉴定出一个214碱基对的缺失。定量实时荧光定量PCR（qRT-PCR）和双亲序列比较分析表明，GhFL10是qFL-c10-1最有希望的候选基因。根据RNA测序、酵母双杂交分析和双分子荧光互补（BiFC），我们推测GhFL10与NF-YA转录因子相互作用，负调控纤维伸长。
