Fang Xiaomei, Liu Xueying, Wang Xiaoqin, Wang Wenwen, Liu Dexin, Zhang Jian, Liu Dajun, Teng Zhonghua, Tan Zhaoyun, Liu Fang, Zhang Fengjiao, Jiang Maochao, Jia Xiuling, Zhong Jianwei, Yang Jinghong, Zhang Zhengsheng
Engineering Research Center of South Upland Agriculture, Ministry of Education, Southwest University, 400716, Chongqing, People's Republic of China.
Theor Appl Genet. 2017 Apr;130(4):795-806. doi: 10.1007/s00122-017-2852-1. Epub 2017 Jan 31.
qFS07.1 controlling fiber strength was fine-mapped to a 62.6-kb region containing four annotated genes. RT-qPCR and sequence of candidate genes identified an LRR RLK gene as the most likely candidate. Fiber strength is an important component of cotton fiber quality and is associated with other properties, such as fiber maturity, fineness, and length. Stable QTL qFS07.1, controlling fiber strength, had been identified on chromosome 7 in an upland cotton recombinant inbred line (RIL) population from a cross (CCRI35 × Yumian1) described in our previous studies. To fine-map qFS07.1, an F population with 2484 individual plants from a cross between recombinant line RIL014 and CCRI35 was established. A total of 1518 SSR primer pairs, including 1062, designed from chromosome 1 of the Gossypium raimondii genome and 456 from chromosome 1 of the G. arboreum genome (corresponding to the QTL region) were used to fine-map qFS07.1, and qFS07.1 was mapped into a 62.6-kb genome region which contained four annotated genes on chromosome A07 of G. hirsutum. RT-qPCR and comparative analysis of candidate genes revealed a leucine-rich repeat protein kinase (LRR RLK) family protein to be a promising candidate gene for qFS07.1. Fine mapping and identification of the candidate gene for qFS07.1 will play a vital role in marker-assisted selection (MAS) and the study of mechanism of cotton fiber development.
控制纤维强度的qFS07.1被精细定位到一个62.6kb的区域，该区域包含四个注释基因。通过实时定量聚合酶链反应（RT-qPCR）和候选基因测序，确定一个富含亮氨酸重复序列的类受体蛋白激酶（LRR RLK）基因是最有可能的候选基因。纤维强度是棉花纤维品质的重要组成部分，并且与其他特性相关，如纤维成熟度、细度和长度。在我们之前的研究中描述的一个陆地棉重组自交系（RIL）群体（CCRI35×豫棉1号杂交组合）中，已在7号染色体上鉴定出稳定的控制纤维强度的QTL qFS07.1。为了精细定位qFS07.1，构建了一个由重组系RIL014与CCRI35杂交产生的包含2484个单株的F群体。总共使用了1518对SSR引物对来精细定位qFS07.1，其中1062对是根据雷蒙德氏棉基因组第1染色体设计的，456对是根据亚洲棉基因组第1染色体（对应于QTL区域）设计的，qFS07.1被定位到陆地棉A07染色体上一个62.6kb的基因组区域，该区域包含四个注释基因。对候选基因进行RT-qPCR和比较分析，发现一个富含亮氨酸重复序列的蛋白激酶（LRR RLK）家族蛋白是qFS07.1很有希望的候选基因。qFS07.1候选基因的精细定位和鉴定将在标记辅助选择（MAS）和棉花纤维发育机制研究中发挥至关重要的作用。
