College of Chemistry, Changchun Normal University, Changchun, 130032, People's Republic of China.
Mikrochim Acta. 2022 Oct 10;189(11):413. doi: 10.1007/s00604-022-05517-w.
A self-enhanced electrochemical luminescence (ECL) composite material g-CN-CdTe QDs was prepared. The combination of g-CN and CdTe QDs can amplify the ECL signal and improve the stability. Based on this discovery, g-CN-CdTe QDs and acetylcholine esterase (AChE) were used to construct an ECL sensor for organophosphorus pesticides (OP) detection. The sensor showed a strong initial ECL signal in PBS containing SO. It is because that g-CN not only acts as a co-reaction promoter to amplify the ECL signal of the CdTe QDs/SO system but also acts as a carrier with large specific surface area to adsorb more CdTe QDs and improve the sensitivity of the sensor. The reaction of AChE and acetylthiocholine (ATCl) was hindered by organophosphorus pesticides (OPs). The ECL signal was enhanced by the addition of OPs, and a linear relationship was displayed between the increasing value and the concentration of malathion. A good linear range from 2.52 × 10 to 2.52 × 10 mol L was obtained and the limit of detection was 8.4 × 10 mol L under optimized experimental conditions. The results indicated that the sensor had promising applications for the detection of OPs in vegetable samples.
一种自增强电化学发光（ECL）复合材料 g-CN-CdTe QDs 被制备。g-CN 和 CdTe QDs 的结合可以放大 ECL 信号并提高稳定性。基于这一发现，g-CN-CdTe QDs 和乙酰胆碱酯酶（AChE）被用于构建用于有机磷农药（OP）检测的 ECL 传感器。该传感器在含有 SO 的 PBS 中显示出强烈的初始 ECL 信号。这是因为 g-CN 不仅作为共反应促进剂来放大 CdTe QDs/SO 体系的 ECL 信号，而且作为具有大比表面积的载体来吸附更多的 CdTe QDs 并提高传感器的灵敏度。AChE 和乙酰硫代胆碱（ATCl）的反应受到有机磷农药（OPs）的阻碍。加入 OPs 会增强 ECL 信号，并且在优化的实验条件下，马拉硫磷的浓度与增加值之间呈现出线性关系。在良好的线性范围内，从 2.52×10 到 2.52×10 mol L 获得了良好的线性范围，检测限为 8.4×10 mol L。结果表明，该传感器在蔬菜样品中 OP 的检测方面具有广阔的应用前景。
