Zarnett R, Delaney J P, Driscoll S W, Salter R B
Clin Orthop Relat Res. 1987 Sep(222):267-74.
A graft of periosteum from the tibia of 27 rabbits was incubated in vitro with tritiated thymidine for 24 hours and then transplanted into a full-thickness defect in the patellar groove. The rabbits were managed after the operation on continuous passive motion (CPM), and the joints excised at intervals of two to 21 days. After one week the cells had begun to synthesize glycosaminoglycan and by two weeks the tissue resembled immature hyaline cartilage. Thymidine-labeled cells were seen throughout the entire regenerated tissue. The cellular origin of the hyaline-like tissue that filled the defects was the progenitor cells of the periosteal graft.
将27只兔子胫骨的骨膜移植物在体外与氚标记的胸腺嘧啶核苷一起孵育24小时，然后移植到髌股沟的全层缺损处。术后对兔子进行持续被动运动（CPM）处理，并在术后2至21天的间隔时间切除关节。一周后，细胞开始合成糖胺聚糖，到两周时，组织类似于未成熟的透明软骨。在整个再生组织中都可见到胸腺嘧啶核苷标记的细胞。填充缺损的类透明软骨组织的细胞来源是骨膜移植物的祖细胞。
