Golikov M V, Valuev-Elliston V T, Smirnova O A, Ivanov A V
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 119991 Moscow, Russia.
Mol Biol. 2022;56(5):629-637. doi: 10.1134/S0026893322050077. Epub 2022 Oct 5.
Changes in cell metabolism accompany the development of a wide spectrum of pathologies including cancer, autoimmune, and inflammatory diseases. Therefore, usage of inhibitors of metabolic enzymes are considered a promising strategy for the development of therapeutic agents. However, the investigation of cellular metabolism is hampered by the significant impact of culture media, which interfere with many cellular processes, thus making cellular models irrelevant. There are numerous reports that show that the results from in vitro systems are not reproduced in in vivo models and patients. Over the last decade a novel approach has emerged, which consists of adaptation of the culture medium composition to that closer to the composition of blood plasma. In 2017‒2019, two plasma-like media were proposed, Plasmax and HPLM. In the review, we have summarized the drawbacks of common media and have analyzed changes in the metabolism of cells cultivated in common and plasma-like media in normal and pathological conditions.
细胞代谢的变化伴随着包括癌症、自身免疫性疾病和炎症性疾病在内的多种病理状况的发展。因此，使用代谢酶抑制剂被认为是开发治疗药物的一种有前景的策略。然而，细胞代谢的研究受到培养基的重大影响的阻碍，培养基会干扰许多细胞过程，从而使细胞模型失去相关性。有大量报告表明，体外系统的结果在体内模型和患者中无法重现。在过去十年中，出现了一种新方法，即调整培养基成分使其更接近血浆成分。在2017年至2019年期间，提出了两种类似血浆的培养基，即Plasmax和HPLM。在这篇综述中，我们总结了常用培养基的缺点，并分析了在正常和病理条件下在常用培养基和类似血浆的培养基中培养的细胞的代谢变化。
