Division of Metabolic Disorders, Department of Internal Medicine, Faculty of Medicine, University of Debrecen, Debrecen, Hungary.
Doctoral School of Health Sciences, Faculty of Public Health, University of Debrecen, Debrecen, Hungary.
Ren Fail. 2022 Dec;44(1):1649-1659. doi: 10.1080/0886022X.2022.2106243.
Pigment epithelium-derived factor (PEDF) is a serin protease inhibitor and a potent inhibitor of angiogenesis. Its serum level has significant associations with metabolic parameters. However, little is known about the association between PEDF levels and lipid parameters in renal transplanted (TX) patients. Therefore, our aim was to investigate the relationship between PEDF level and lipid parameters in TX patients.
Seventy TX patients (47 males, 23 females, mean age 51.7 ± 12.4 years) and 34 healthy controls were enrolled. We examined the serum creatinine, C-reactive protein, fasting glucose and lipid parameters right before, then 1 and 6 months after TX. High-density lipoprotein (HDL)-associated paraoxonase-1 (PON1) activities were measured spectrophotometrically. Lipoprotein subfractions were determined by Lipoprint. PEDF and oxidized low-density liporotein (oxLDL) levels were measured by ELISA.
Before transplantation, patients had had a significantly higher PEDF level compared to control subjects ( < 0.001). One month after transplantation, their PEDF level decreased significantly reaching the healthy controls' level, and this lower level was maintained during the 6 months follow-up period as well. The initial oxLDL level was significantly higher, while PON1 activities were significantly lower in the patient group compared to the control group. We found a significant positive correlation between PEDF and total cholesterol, low-density lipoprotein (LDL)-cholesterol, triglyceride, oxLDL and small HDL subfraction; while negative correlations were found between PEDF and mean LDL size and large HDL subfraction during the entire follow-up period.
PEDF may play an important role in the increased oxidative stress and enhanced atherogenesis in renal transplant patients.
色素上皮衍生因子（PEDF）是一种丝氨酸蛋白酶抑制剂，也是血管生成的有效抑制剂。其血清水平与代谢参数有显著关联。然而，关于 PEDF 水平与肾移植（TX）患者脂质参数之间的关系知之甚少。因此，我们的目的是研究 TX 患者 PEDF 水平与脂质参数之间的关系。
纳入 70 名 TX 患者（47 名男性，23 名女性，平均年龄 51.7±12.4 岁）和 34 名健康对照者。我们在 TX 前、TX 后 1 个月和 6 个月时检查了血清肌酐、C 反应蛋白、空腹血糖和脂质参数。采用分光光度法测定高密度脂蛋白（HDL）相关对氧磷酶-1（PON1）活性。通过 Lipoprint 测定脂蛋白亚组分。采用 ELISA 法测定 PEDF 和氧化型低密度脂蛋白（oxLDL）水平。
移植前，患者的 PEDF 水平明显高于对照组（<0.001）。移植后 1 个月，其 PEDF 水平显著下降，达到健康对照组水平，并在 6 个月随访期间保持较低水平。与对照组相比，患者组的初始 oxLDL 水平显著升高，而 PON1 活性显著降低。我们发现 PEDF 与总胆固醇、低密度脂蛋白（LDL）-胆固醇、甘油三酯、oxLDL 和小 HDL 亚组分呈显著正相关；而在整个随访期间，PEDF 与 LDL 平均大小和大 HDL 亚组分呈负相关。
PEDF 可能在肾移植患者氧化应激增加和动脉粥样硬化形成增强中发挥重要作用。
