Oud J L, Peters P W
Mutat Res. 1978 Oct;54(2):175-84. doi: 10.1016/0165-1161(78)90038-9.
A sequence is described of test procedures for a screening in vivo of the clastogenic potential of the alkylating agent triaziquone (Trenimon). Two intraperitoneal injections of 0.125 mg/kg body weight caused a considerable increase in the number of aberrations in both the micronucleus test and the bone-marrow metaphase test, but not in the spermatocyte translocation test or the spermatogonial metaphase test. With the latter test a severe cell-killing effect was detected. An analysis of whole mounts of seminiferous tubules showed that 0.125 mg/kg was a lethal dose for all B- and intermediate-type spermatogonia and partly killed A-type spermatogonia. A single administration of the same dose caused stable chromosomal rearrangements in spermatids that could be demonstrated with the F1 translocation test, and gave rise to dominant lethality of fetuses originating from post-meiotic sperm. The comparative triaziquone study has provided arguments in favor of the micronucleus test as a reliable screening method for chromosomal aberrations. The analysis of seminiferous tubules is a recommendable method for studying lethal effects of a compound on germ cells, whereas the F1 translocation test gives important information about viable aberrations and their effect on the fertility of the progeny.
本文描述了一系列用于体内筛选烷化剂三亚胺醌（癌抑散）致断裂潜力的测试程序。两次腹腔注射0.125mg/kg体重剂量的三亚胺醌，在微核试验和骨髓中期试验中均导致畸变数量显著增加，但在精母细胞易位试验或精原细胞中期试验中未出现这种情况。在后者的试验中检测到了严重的细胞杀伤作用。对生精小管整装片的分析表明，0.125mg/kg对所有B型和中间型精原细胞都是致死剂量，并且部分杀死了A型精原细胞。单次给予相同剂量可导致精子细胞出现稳定的染色体重排，这可通过F1易位试验得到证实，并导致减数分裂后精子来源的胎儿出现显性致死。三亚胺醌的比较研究为微核试验作为一种可靠的染色体畸变筛选方法提供了依据。生精小管分析是研究化合物对生殖细胞致死作用的一种推荐方法，而F1易位试验则提供了有关存活畸变及其对后代生育力影响的重要信息。
