Oud J L, de Jong J H, de Rooij D G
Chromosoma. 1979 Feb 21;71(2):237-48. doi: 10.1007/BF00292826.
A method is described to restrict the spermatocyte population in mice and other rodents using hydroxyurea (HU) and triaziquone (T). HU affects cells in S-phase, whereas T is an agent especially active on spermatogonia and not on spermatocytes. An application of three i.p. HU injections with 12 h intervals, followed about nine days later by one i.p. T injection creates two large gaps in the spermatogenic line. The two gaps enclose a small, well-defined group of primary spermatocytes in meiotic interphase. - The development of the restricted spermatocyte population is followed day by day. The analysis of meiosis in male mice has revealed the correct sequence of meiotic, and especially prophase I stages. On account of clearly visible differences in chromosome morphology the diplotene stage could be divided into three periods. It is suggested to use the following nomenclature: pre-diffuse diplotene, diffuse diplotene and post-difuse diplotene. The experiment was also informative about the timing of the stages in spermatocyte development by correlating the days at which the successive stages were observed with the corresponding stage of the epithelial cycle. The calculation of the position and duration of the diffuse diplotene, enables us to put forward a proposal about the significance of the diffuse diplotene. - A combination of the HU/T method with cell separation techniques provides good perspectives for detailed biochemical studies on processes taking place during meiosis.
本文描述了一种使用羟基脲（HU）和三嗪醌（T）来限制小鼠和其他啮齿动物精母细胞群体的方法。HU影响处于S期的细胞，而T是一种对精原细胞特别有活性而对精母细胞无活性的试剂。腹腔注射三次HU，间隔12小时，然后大约九天后腹腔注射一次T，会在生精谱系中产生两个大的间隙。这两个间隙包围着一小群处于减数分裂间期的明确界定的初级精母细胞。——对受限精母细胞群体的发育进行逐日跟踪。对雄性小鼠减数分裂的分析揭示了减数分裂，尤其是前期I各阶段的正确顺序。由于染色体形态存在明显可见的差异，双线期可分为三个时期。建议使用以下命名法：前弥散双线期、弥散双线期和后弥散双线期。通过将观察到连续阶段的天数与上皮周期的相应阶段相关联，该实验还提供了有关精母细胞发育各阶段时间安排的信息。弥散双线期位置和持续时间的计算，使我们能够对弥散双线期的意义提出一项建议。——HU/T方法与细胞分离技术的结合为减数分裂过程中发生的过程的详细生化研究提供了良好的前景。
