兔出血症病毒 2 型的早期发病机制。
Early pathogenesis in rabbit hemorrhagic disease virus 2.
机构信息
Department of Pathology, College of Veterinary Medicine, University of Georgia, Athens, GA, USA.
United States Department of Agriculture, Animal and Plant Health Inspection Service, Foreign Animal Disease Diagnostic Laboratory, Plum Island Animal Disease Center, Greenport, NY, USA.
出版信息
Microb Pathog. 2022 Dec;173(Pt A):105814. doi: 10.1016/j.micpath.2022.105814. Epub 2022 Oct 8.
To detail early tissue distribution and innate immune response to rabbit hemorrhagic disease virus 2 (RHDV2), 13 rabbits were orally (Oryctolagus cuniculus) inoculated with liver homogenate made from a feral rabbit that succumbed to RHDV2 during the 2020 outbreak in Oregon, USA. Rabbits were monitored regularly, with euthanasia and collection of tissues and swabs, at 12, 24, 36, 48, 96, and 144 h post inoculation. Livers from these rabbits were positive by RT-rtPCR for presence of the virus. Using RNAscope for viral and replicative intermediates, rabbits had detectable viral genomic RNA at each time point, initially within the gastrointestinal tract, then in the liver by 36 h post inoculation. Also using RNAscope, there were increasing amounts of mRNA coding for TNF-α, IL-6, and IL-1β within the liver and spleen through 48 h post inoculation. The results of this study aided our understanding of the local innate immune response to RHDV2, as well as aspects of pathogenesis.
为详细了解兔出血症病毒 2 型（RHDV2）在早期的组织分布和固有免疫反应，我们对 13 只家兔进行了经口（Oryctolagus cuniculus）接种，所用肝匀浆取自美国俄勒冈州 2020 年暴发疫情中一只死于 RHDV2 的野生兔。接种后 12、24、36、48、96 和 144 小时，定期监测家兔并实施安乐死，采集组织和拭子。这些兔子的肝脏通过 RT-rtPCR 检测到病毒存在。使用 RNAscope 检测病毒和复制中间体，结果显示在每个时间点，家兔均检测到病毒基因组 RNA，最初在胃肠道内，然后在接种后 36 小时在肝脏内。同样使用 RNAscope，在接种后 48 小时内，肝脏和脾脏内 TNF-α、IL-6 和 IL-1β 的编码 mRNA 量逐渐增加。本研究结果有助于我们了解 RHDV2 的局部固有免疫反应以及发病机制的某些方面。
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