Wang Fei, Zou Peng, Xu Shujie, Wang Qi, Zhou Yuanhao, Li Xiang, Tang Li, Wang Baikui, Jin Qian, Yu Dongyou, Li Weifen
Key Laboratory of Animal Molecular Nutrition of Education of Ministry, National Engineering Laboratory of Biological Feed Safety and Pollution Prevention and Control, Key Laboratory of Animal Feed and Nutrition of Zhejiang Province, Institute of Animal Nutrition and Feed Sciences, College of Animal Sciences, Zhejiang University, Hangzhou, 310058, China.
Hainan Institute, Zhejiang University, Yazhou Bay Sci-Tech City, Yongyou Industry Park, Sanya, 572000, China.
J Anim Sci Biotechnol. 2022 Oct 13;13(1):118. doi: 10.1186/s40104-022-00766-4.
This study aimed to investigate whether the combination of Macleaya cordata extract (MCE) and Bacillus could improve the laying performance and health of laying hens better.
A total of 360 29-week-old Jingbai laying hens were randomly divided into 4 treatments: control group (basal diet), MCE group (basal diet + MCE), Probiotics Bacillus Compound (PBC) group (basal diet + compound Bacillus), MCE + PBC group (basal diet + MCE + compound Bacillus). The feeding experiment lasted for 42 d.
The results showed that the laying rate and the average daily egg mass in the MCE + PBC group were significantly higher than those in the control group (P < 0.05) and better than the MCE and PBC group. Combination of MCE and Bacillus significantly increased the content of follicle-stimulating hormone (FSH) in the serum and up-regulated the expression of related hormone receptor gene (estrogen receptor-β, FSHR and luteinizing hormone/choriogonadotropin receptor) in the ovary of laying hens (P < 0.05). In the MCE + PBC group, the mRNA expressions of zonula occluden-1, Occludin and mucin-2 in jejunum was increased and the intestinal epithelial barrier detected by transmission electron microscopy was enhanced compared with the control group (P < 0.05). In addition, compared with the control group, combination of MCE and Bacillus significantly increased the total antioxidant capacity and catalase activity (P < 0.05), and down-regulated the mRNA expressions of inflammation-related genes (interleukin-1β and tumor necrosis factor-α) as well as apoptosis-related genes (Caspase 3, Caspase 8 and P53) (P < 0.05). The concentration of acetic acid and butyric acid in the cecum content of laying hens in the MCE + PBC group was significantly increased compared with the control group (P < 0.05).
Collectively, dietary supplementation of 600 μg/kg MCE and 5 × 10 CFU/kg compound Bacillus can improve laying performance by improving microbiota to enhance antioxidant capacity and intestinal barrier, regulate reproductive hormones and the concentration of cecal short-chain fatty acids of laying hens, and the combined effect of MCE and Bacillus is better than that of single supplementation.
本研究旨在探讨博落回提取物（MCE）与芽孢杆菌联合使用是否能更好地提高蛋鸡的产蛋性能和健康水平。
将360只29周龄的京白蛋鸡随机分为4组：对照组（基础日粮）、MCE组（基础日粮+MCE）、益生菌芽孢杆菌复合组（PBC组，基础日粮+复合芽孢杆菌）、MCE+PBC组（基础日粮+MCE+复合芽孢杆菌）。饲养试验持续42天。
结果表明，MCE+PBC组的产蛋率和平均日产蛋量显著高于对照组（P<0.05），且优于MCE组和PBC组。MCE与芽孢杆菌联合使用显著提高了血清中促卵泡激素（FSH）的含量，并上调了蛋鸡卵巢中相关激素受体基因（雌激素受体-β、FSHR和促黄体激素/绒毛膜促性腺激素受体）的表达（P<0.05）。与对照组相比，MCE+PBC组空肠中紧密连接蛋白-1、闭合蛋白和黏蛋白-2的mRNA表达增加，透射电子显微镜检测的肠上皮屏障增强（P<0.05）。此外，与对照组相比，MCE与芽孢杆菌联合使用显著提高了总抗氧化能力和过氧化氢酶活性（P<0.05），并下调了炎症相关基因（白细胞介素-1β和肿瘤坏死因子-α）以及凋亡相关基因（半胱天冬酶3、半胱天冬酶8和P53）的mRNA表达（P<0.05）。MCE+PBC组蛋鸡盲肠内容物中乙酸和丁酸的浓度显著高于对照组（P<0.05）。
总体而言，日粮中添加600μg/kg MCE和5×10CFU/kg复合芽孢杆菌可通过改善微生物群以增强抗氧化能力和肠道屏障、调节蛋鸡生殖激素和盲肠短链脂肪酸浓度来提高产蛋性能，且MCE与芽孢杆菌的联合效果优于单一添加。
