普雷沃氏菌和琥珀酸盐处理改变了蛋鸡的肠道微生物群，提高了产蛋性能，并抑制了肝脏脂质积累。

Prevotella and succinate treatments altered gut microbiota, increased laying performance, and suppressed hepatic lipid accumulation in laying hens.

作者信息

Liu Min, Kang Zeyue, Cao Xikang, Jiao Hongchao, Wang Xiaojuan, Zhao Jingpeng, Lin Hai

机构信息

College of Animal Science and Technology, Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Key Laboratory of Efficient Utilization of Non-Grain Feed Resources (Co-Construction by Ministry and Province), Ministry of Agriculture and Rural Affairs, Shandong Agricultural University, Tai'an, 271018, China.

出版信息

J Anim Sci Biotechnol. 2024 Feb 18;15(1):26. doi: 10.1186/s40104-023-00975-5.

DOI:10.1186/s40104-023-00975-5

PMID:38369510

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10874536/

Abstract

BACKGROUND

This work aimed to investigate the potential benefits of administering Prevotella and its primary metabolite succinate on performance, hepatic lipid accumulation and gut microbiota in laying hens.

RESULTS

One hundred and fifty 58-week-old Hyline Brown laying hens, with laying rate below 80% and plasma triglyceride (TG) exceeding 5 mmol/L, were used in this study. The hens were randomly allocated into 5 groups and subjected to one of the following treatments: fed with a basal diet (negative control, NC), oral gavage of 3 mL/hen saline every other day (positive control, PC), gavage of 3 mL/hen Prevotella melaninogenica (10 CFU/mL, PM) or 3 mL/hen Prevotella copri (10 CFU/mL, P. copri) every other day, and basal diet supplemented with 0.25% sodium succinate (Succinate). The results showed that PM and P. copri treatments significantly improved laying rate compared to the PC (P < 0.05). The amount of lipid droplet was notably decreased by PM, P. copri, and Succinate treatments at week 4 and decreased by P. copri at week 8 (P < 0.05). Correspondingly, the plasma TG level in Succinate group was lower than that of PC (P < 0.05). Hepatic TG content, however, was not significantly influenced at week 4 and 8 (P > 0.05). PM treatment increased (P < 0.05) the mRNA levels of genes PGC-1β and APB-5B at week 4, and ACC and CPT-1 at week 8. The results indicated enhanced antioxidant activities at week 8, as evidenced by reduced hepatic malondialdehyde (MDA) level and improved antioxidant enzymes activities in PM and Succinate groups (P < 0.05). Supplementing with Prevotella or succinate can alter the cecal microbiota. Specifically, the abundance of Prevotella in the Succinate group was significantly higher than that in the other 4 groups at the family and genus levels (P < 0.05).

CONCLUSIONS

Oral intake of Prevotella and dietary supplementation of succinate can ameliorate lipid metabolism of laying hens. The beneficial effect of Prevotella is consistent across different species. The finding highlights that succinate, the primary metabolite of Prevotella, represents a more feasible feed additive for alleviating fatty liver in laying hens.

摘要

背景

本研究旨在探讨给予普雷沃氏菌及其主要代谢产物琥珀酸盐对蛋鸡生产性能、肝脏脂质积累和肠道微生物群的潜在益处。

结果

本研究选用150只58周龄海兰褐蛋鸡，产蛋率低于80%且血浆甘油三酯（TG）超过5 mmol/L。将母鸡随机分为五组，分别进行以下处理之一：饲喂基础日粮（阴性对照，NC）、每隔一天每只鸡口服灌胃3 mL生理盐水（阳性对照，PC）、每隔一天每只鸡灌胃3 mL产黑素普雷沃氏菌（10⁸ CFU/mL，PM）或3 mL普通普雷沃氏菌（10⁸ CFU/mL，P. copri），以及基础日粮中添加0.25%琥珀酸钠（琥珀酸盐）。结果表明，与PC组相比，PM和P. copri处理显著提高了产蛋率（P < 0.05）。在第4周，PM组、P. copri组和琥珀酸盐组的脂滴数量显著减少，在第8周，P. copri组的脂滴数量减少（P < 0.05）。相应地，琥珀酸盐组的血浆TG水平低于PC组（P < 0.05）。然而，在第4周和第8周，肝脏TG含量没有受到显著影响（P > 0.05）。PM处理在第4周增加了（P < 0.05）PGC - 1β和APB-5B基因的mRNA水平，在第8周增加了ACC和CPT-1基因的mRNA水平。结果表明，在第8周抗氧化活性增强，这在PM组和琥珀酸盐组中表现为肝脏丙二醛（MDA）水平降低和抗氧化酶活性提高（P < 0.05）。补充普雷沃氏菌或琥珀酸盐可以改变盲肠微生物群。具体而言，在科和属水平上，琥珀酸盐组中的普雷沃氏菌丰度显著高于其他4组（P < 0.05）。