Khodakaram-Tafti A, Derakhshandeh A, Daee A A, Seyedin M
Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
Ph.D. Student in Veterinary Pathology, Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
Iran J Vet Res. 2023;24(2):96-101. doi: 10.22099/IJVR.2023.45321.6655.
A number of spp., often referred to as the (Mm) cluster can produce respiratory tract infections in goats; however, only subspecies (Mccp) is considered to causecontagious caprine pleuropneumonia.
Isolation and identification of subspecies and from the pneumonic lungs of slaughtered goats and their association with pathological changes.
Lungs of 2000 goats slaughtered at an industrial abattoir in Mashhad, Iran, were examined for the presence of gross pneumonic lesions. Fifty affected lungs were selected for pathology, culture, and molecular (PCR) studies for the presence of species. DNA was extracted from lung tissue samples and replicated using genus and species specific primers for .
Grossly, consolidation and dark red to grey discoloration in the cranioventral to caudal lobes in fibrinopurulent bronchopneumonia and rubbery texture associated with rib impressions on the costal surfaces of the diaphragmatic lobes in interstitial pneumonia were observed. Histopathologically, bronchointerstitial pneumonia in 40 (80%), and fibrinopurulent bronchopneumonia in 10 (20%) of affected goats were diagnosed. The evidence of growth such as turbidity and colonies on the agar plates was observed in 2 (4%) of samples. Genus-specific DNA was identified in 11 (22%) of samples. Of them, 3 (6%) and 3 (6%) of tissue lung samples were positive for subspecies and , respectively, by PCR.
Our results showed that subspecies and were the two agents that can involve lung consolidation and pneumonia in goats.
多个丝状支原体物种，通常被称为丝状支原体簇，可在山羊中引起呼吸道感染；然而，只有丝状支原体山羊亚种（Mccp）被认为会引发山羊传染性胸膜肺炎。
从屠宰山羊的肺炎肺组织中分离和鉴定丝状支原体山羊亚种和丝状支原体，并研究它们与病理变化的关系。
对在伊朗马什哈德一家工业屠宰场屠宰的2000只山羊的肺进行检查，查看是否存在明显的肺炎病变。选取50个受影响的肺组织进行病理学、培养和分子（PCR）研究，以检测丝状支原体物种的存在。从肺组织样本中提取DNA，并使用丝状支原体属和种特异性引物进行扩增。
肉眼观察，在纤维素性脓性支气管肺炎中，可见从颅腹侧到尾叶的实变以及暗红色至灰色变色，在间质性肺炎中，可见膈叶肋面有与肋骨压痕相关的橡胶样质地。组织病理学诊断显示，40只（80%）受影响山羊为支气管间质性肺炎，10只（20%）为纤维素性脓性支气管肺炎。在2个（4%）样本中观察到在绵羊鲜血琼脂平板上有丝状支原体生长的迹象，如浑浊和菌落。在11个（22%）样本中鉴定出丝状支原体属特异性DNA。其中，通过PCR检测，3个（6%）肺组织样本为丝状支原体山羊亚种阳性，3个（6%）为丝状支原体阳性。
我们的结果表明，丝状支原体山羊亚种和丝状支原体是可导致山羊肺部实变和肺炎的两种病原体。
