用于连续飞秒晶体学实验的 RNA 微晶的制备。

Preparation of RNA Microcrystals for Serial Femtosecond Crystallography Experiments.

机构信息

Protein-Nucleic Acid Interaction Section, Center for Structural Biology, Center for Cancer Research, National Cancer Institute, Frederick, MD, USA.

出版信息

Methods Mol Biol. 2023;2568:233-242. doi: 10.1007/978-1-0716-2687-0_15.

DOI:10.1007/978-1-0716-2687-0_15

PMID:36227572

Abstract

Serial femtosecond crystallography (SFX) experiments using an X-ray free electron laser (XFEL) is a burgeoning method for time-resolved structural studies of biomacromolecules. As with any crystallography experiment, the most important component is quality sample preparation. Whereas dozens of SFX experiments, including batch crystallization methods, have been reported for proteins, very few have been reported for RNA. This chapter outlines standard procedures for preparing RNA microcrystalline samples suitable for SFX studies.

摘要

利用 X 射线自由电子激光（XFEL）进行连续的飞秒晶体学（SFX）实验是一种新兴的生物大分子时间分辨结构研究方法。与任何晶体学实验一样，最重要的组成部分是高质量的样品制备。尽管已经有数十项 SFX 实验（包括批量结晶方法）被报道用于蛋白质，但很少有用于 RNA 的报道。本章概述了适用于 SFX 研究的 RNA 微晶体样品制备的标准程序。