Kupitz Christopher, Grotjohann Ingo, Conrad Chelsie E, Roy-Chowdhury Shatabdi, Fromme Raimund, Fromme Petra
Department of Chemistry and Biochemistry, Arizona State University, Tempe, AZ 85281, USA.
Department of Chemistry and Biochemistry, Arizona State University, Tempe, AZ 85281, USA
Philos Trans R Soc Lond B Biol Sci. 2014 Jul 17;369(1647):20130316. doi: 10.1098/rstb.2013.0316.
Serial femtosecond crystallography (SFX) is a new emerging method, where X-ray diffraction data are collected from a fully hydrated stream of nano- or microcrystals of biomolecules in their mother liquor using high-energy, X-ray free-electron lasers. The success of SFX experiments strongly depends on the ability to grow large amounts of well-ordered nano/microcrystals of homogeneous size distribution. While methods to grow large single crystals have been extensively explored in the past, method developments to grow nano/microcrystals in sufficient amounts for SFX experiments are still in their infancy. Here, we describe and compare three methods (batch, free interface diffusion (FID) and FID centrifugation) for growth of nano/microcrystals for time-resolved SFX experiments using the large membrane protein complex photosystem II as a model system.
串行飞秒晶体学（SFX）是一种新兴方法，它利用高能X射线自由电子激光，从母液中完全水合的生物分子纳米或微晶的连续流中收集X射线衍射数据。SFX实验的成功很大程度上取决于生长大量尺寸分布均匀的有序纳米/微晶的能力。虽然过去已经广泛探索了生长大单晶的方法，但开发出足够数量用于SFX实验的纳米/微晶的方法仍处于起步阶段。在此，我们描述并比较了三种用于生长纳米/微晶的方法（批量法、自由界面扩散（FID）法和FID离心法），这些方法用于以大型膜蛋白复合物光系统II作为模型系统的时间分辨SFX实验。
