Bruns C, Schächtele C, Marmé D
FEBS Lett. 1987 Aug 31;221(1):23-7. doi: 10.1016/0014-5793(87)80345-9.
The two activators of protein kinase C, oleoylacetylglycerol (OAG) and dioctanoylglycerol (DOG), are able to induce a concentration-dependent rise in cytoplasmic free Ca2+ concentration in gel-filtered human platelets, detected as an increase in quin2 fluorescence. The phorbol ester phorbol-12-myristate-13-acetate (PMA) has no effect. The OAG-induced increase of intracellular Ca2+ is not influenced by forskolin, in contrast to the effect of the diterpene on the thrombin-stimulated increase in cytoplasmic Ca2+. It is concluded that the increase in intracellular free Ca2+ concentration induced by synthetic diacylglycerols and their activation of protein kinase C are two different and independent processes.
蛋白激酶C的两种激活剂，油酰乙酰甘油（OAG）和二辛酰甘油（DOG），能够在凝胶过滤的人血小板中诱导细胞质游离钙离子浓度呈浓度依赖性升高，这通过喹啉2荧光增加得以检测。佛波酯佛波醇-12-肉豆蔻酸酯-13-乙酸酯（PMA）无此作用。与二萜对凝血酶刺激引起的细胞质钙离子增加的作用相反，OAG诱导的细胞内钙离子增加不受福斯高林影响。得出的结论是，合成二酰甘油诱导的细胞内游离钙离子浓度增加及其对蛋白激酶C的激活是两个不同且独立的过程。
