前列腺素I2和福斯高林对凝血酶、胶原蛋白、佛波酯和外源性二酰基甘油在细胞质游离钙基础浓度下诱发的血小板分泌的影响。
Effects of prostaglandin I2 and forskolin on the secretion from platelets evoked at basal concentrations of cytoplasmic free calcium by thrombin, collagen, phorbol ester and exogenous diacylglycerol.
作者信息
Rink T J, Sanchez A
出版信息
Biochem J. 1984 Sep 15;222(3):833-6. doi: 10.1042/bj2220833.
Abstract
Cytoplasmic free calcium ([Ca2+]i) and secretion of ATP were measured in quin2-loaded human platelets. In certain conditions thrombin and collagen cause secretion while [Ca2+]i remains at basal concentrations, a response attributed to activation of protein kinase by diacylglycerol formed by hydrolysis of inositol lipids. This secretion evoked by thrombin could be totally suppressed by prostaglandin I2 or forskolin, as expected from the known ability of cyclic AMP to inhibit phospholipase C. The secretory response evoked by collagen at basal [Ca2+]i and that evoked by exogenous diacylglycerol or phorbol ester, direct activators of protein kinase-C, were much less affected by these inhibitors, suggesting that thrombin and collagen may promote formation of diacylglycerol by different mechanisms.
摘要
在负载喹啉-2的人血小板中测量细胞质游离钙（[Ca2+]i）和ATP分泌。在某些条件下，凝血酶和胶原蛋白会引起分泌，而[Ca2+]i保持在基础浓度，这种反应归因于由肌醇脂质水解形成的二酰基甘油对蛋白激酶的激活。如预期的那样，环磷酸腺苷抑制磷脂酶C的已知能力表明，凝血酶引起的这种分泌可被前列腺素I2或福斯高林完全抑制。在基础[Ca2+]i水平下，胶原蛋白引起的分泌反应以及由二酰基甘油或佛波酯（蛋白激酶C的直接激活剂）引起的分泌反应受这些抑制剂的影响要小得多，这表明凝血酶和胶原蛋白可能通过不同机制促进二酰基甘油的形成。
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Calcium homeostasis in intact lymphocytes: cytoplasmic free calcium monitored with a new, intracellularly trapped fluorescent indicator.完整淋巴细胞中的钙稳态：用一种新的细胞内捕获荧光指示剂监测细胞质游离钙。
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Inositol lipid metabolism in the responses of stimulated platelets.刺激血小板反应中的肌醇脂质代谢
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