多基因共表达改变了睾丸生长、激素分泌和精子发生，促使湖羊提前性成熟。

Polygenic co-expression changes the testis growth, hormone secretion and spermatogenesis to prompt puberty in Hu sheep.

机构信息

Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China; Laboratory of Sheep and Goat Genetics, Breeding and Reproduction, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.

出版信息

Theriogenology. 2022 Dec;194:116-125. doi: 10.1016/j.theriogenology.2022.09.025. Epub 2022 Oct 4.

DOI:10.1016/j.theriogenology.2022.09.025

PMID:36228521

Abstract

In most male mammals, testis undergoes increased proliferation activity and the onset of spermatogenesis during pubertal development. However, their gene expression patterns and roles in sheep remain unclear. Therefore, we used Illumina Hiseq 2000 sequencing to identify differentially expressed genes (DEGs) from 0, 30, 60, 90, 120, 150 and 180 postnatal days and characterize the transcriptional level of sheep testicular development. Among them, the DEGs changed the most in 90-150 stages. a total of 2546 (1454 up and 1092 down) and 6867 (4683 up and 2184 down) DEGs were identified in D120 vs. D90 and D150 vs. D120, respectively. Functional enrichment analysis revealed that in earlier pubertal development, testis showed higher gene expression in organ morphogenesis, vasculogenesis, neurogenesis and hormone secretion, while in later pubertal development, genes with higher expression mainly concentrated in regulating spermatogenesis process. These results indicated that testis development undergoes the transition from organ growth to spermatogenesis and the genes related to hormone secretion were expressed highly earlier than spermatogenesis during pubertal process. In addition, we found several genes such as ZFP36, TNF, HSD3B1, HSD11B2 played key roles in androgen secretion and SPAG family, SYCP family, SPATA family, SPO11, CABYR, TNP1, TNP2 and CFAP43 performed functions during spermatogenesis process. Taken together, multi-genes cooperation prompt testis development in pubertal process. Our transcriptional atlas of sheep testis provides a comprehensive insight about testicular development and pubertal process.

摘要

在大多数雄性哺乳动物中，睾丸在青春期发育过程中经历增殖活动的增加和精子发生的开始。然而，它们的基因表达模式和在绵羊中的作用尚不清楚。因此，我们使用 Illumina Hiseq 2000 测序来鉴定 0、30、60、90、120、150 和 180 日龄绵羊睾丸发育过程中的差异表达基因（DEGs），并描述绵羊睾丸发育的转录水平。其中，90-150 阶段的 DEGs 变化最大。在 D120 与 D90 相比和 D150 与 D120 相比中，分别鉴定到 2546 个（1454 个上调和 1092 个下调）和 6867 个（4683 个上调和 2184 个下调）DEGs。功能富集分析表明，在早期青春期发育中，睾丸在器官形态发生、血管生成、神经发生和激素分泌方面表现出更高的基因表达，而在后期青春期发育中，高表达的基因主要集中在调节精子发生过程上。这些结果表明，睾丸发育经历从器官生长到精子发生的转变，并且在青春期过程中，与激素分泌相关的基因表达早于精子发生。此外，我们发现了一些基因，如 ZFP36、TNF、HSD3B1、HSD11B2，它们在雄激素分泌中发挥关键作用，而 SPAG 家族、SYCP 家族、SPATA 家族、SPO11、CABYR、TNP1、TNP2 和 CFAP43 在精子发生过程中发挥作用。总之，多种基因的合作促使睾丸在青春期发育。我们的绵羊睾丸转录图谱提供了对睾丸发育和青春期过程的全面了解。