巴西潘帕斯蛇（Bothrops pubescens）毒液的自上而下和自下而上蛋白质组学特征分析。
Venom characterization of the Brazilian Pampa snake Bothrops pubescens by top-down and bottom-up proteomics.
机构信息
Applied Proteomics Laboratory, Federal University of Pampa, São Gabriel, Brazil; Pos Graduation in Biological Sciences, Federal University of Pampa, São Gabriel, Brazil.
Departments of Molecular Biosciences, Chemistry, and the Feinberg School of Medicine, Northwestern University, Evanston, IL, 60208, United States.
出版信息
Toxicon. 2022 Dec;220:106937. doi: 10.1016/j.toxicon.2022.106937. Epub 2022 Oct 10.
Abstract
The envenomation from the Bothrops genus is characterized by systemic and local effects caused by the main toxin families in the venom. In Bothrops pubescens venom we were able to identify 89 protein groups belonging to 13 toxin families with the bottom-up proteomics approach and 40 unique proteoforms belonging to 6 toxin families with the top-down proteomics approach. We also identified multi-proteoform complexes of dimeric L-amino acid oxidase using native top-down mass spectrometry.
摘要
该属的蛇毒中毒的特点是由毒液中的主要毒素家族引起的全身和局部作用。在 B. pubescens 毒液中，我们使用 bottom-up 蛋白质组学方法鉴定了属于 13 个毒素家族的 89 个蛋白质组，使用 top-down 蛋白质组学方法鉴定了属于 6 个毒素家族的 40 个独特的蛋白质组。我们还使用天然 top-down 质谱法鉴定了二聚 L-氨基酸氧化酶的多蛋白质组复合物。
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