Graduate Program in Biotechnology, State University of Feira de Santana (UEFS), Feira de Santana, BA, Brazil.
Graduate Program in Tropical Diseases, Botucatu Medical School (FMB), São Paulo State University (UNESP), Botucatu, SP, Brazil.
Toxicon. 2022 Jul 15;213:27-42. doi: 10.1016/j.toxicon.2022.04.002. Epub 2022 Apr 8.
Bothrops leucurus is considered as a snake of medical interest in the State of Bahia, Brazil. However, so far, there are no studies that provide a refined mapping of the composition of this venom. The aim of this work was to better understand the protein composition of B. leucurus snake venom and to isolate and biologically characterize the most abundant toxin, a basic PLA-like. Shotgun proteomics approach identified 137 protein hits in B. leucurus venom subdivided into 19 protein families. The new basic PLAlike toxin identified was denominated Bleu-PLA-like, it and other proteoforms represents about 25% of the total proteins in the venom of B. leucurus and induces myotoxicity, inflammation and muscle damage. Immunoreactivity assays demonstrated that B. leucurus venom is moderately recognized by bothropic and crotalic antivenoms, and on the other hand, Bleu-PLA-like and its proteoforms are poorly recognized. Our findings open doors for future studies in order to assess the systemic effects caused by this snake venom in order to better understand the toxinological implications of this envenomation and, consequently, to assist in the clinical treatment of victims.
巴西巴伊亚州将矛头蝮（Bothrops leucurus）视为具有医学重要性的蛇类。然而，迄今为止，尚无研究对这种毒液的组成进行精细描绘。本研究旨在更好地了解矛头蝮蛇毒液的蛋白质组成，并分离和生物学表征最丰富的毒素，即一种碱性 PLA2 样毒素。Shotgun 蛋白质组学方法鉴定了 B. leucurus 毒液中的 137 种蛋白，分为 19 种蛋白家族。鉴定出的新型碱性 PLA2 样毒素命名为 Bleu-PLA-like，它和其他蛋白异构体约占 B. leucurus 毒液总蛋白的 25%，并具有肌毒性、炎症和肌肉损伤作用。免疫反应性测定表明，矛头蝮和响尾蛇抗蛇毒血清对 B. leucurus 毒液的识别程度中等，而 Bleu-PLA-like 及其蛋白异构体的识别程度较差。我们的研究结果为进一步研究这种蛇毒引起的全身效应开辟了道路，以便更好地了解这种蛇毒的毒理学意义，并为受害者的临床治疗提供帮助。
