Galau G A, Hughes D W
Dev Biol. 1987 Sep;123(1):213-21. doi: 10.1016/0012-1606(87)90443-x.
One of two related patterns of total transcript accumulation are seen during embryogenesis for 18 cotton Lea (Late embryogenesis-abundant) gene families in the allotetraploid cotton Gossypium hirsutum L. cv Coker 201. Coordinate accumulation in each class is complex, suggesting that Lea mRNA abundance is regulated by several events. Each of the Lea gene families probably contains two active homeologous genes (alloalleles), one in each of cotton's two subgenomes. It is of interest whether both transcripts of a Lea family are regulated the same or whether the complexity of total transcript accumulation is due to different regulation of the two transcripts. The two polypeptides encoded by the homeologous transcripts of 7 Lea families can be distinguished on two-dimensional gels. The majority of Lea transcripts in total RNAs is shown to be functional in vitro throughout development; thus in vitro translation should faithfully measure their relative abundance. The ratio of the two transcripts of each was followed during embryo maturation when Lea transcript concentration increases an average of 70-fold and during the first 12 hr of germination when Lea transcripts decline in concentration an average of 50-fold. For 6 of the Lea families, the relative level of the two transcripts is invariant throughout this period. The two constituent transcripts of the seventh, Lea9, change 5-fold in relative concentration during late maturation and 3-fold during germination. Both transcripts still follow the same temporal pattern of accumulation; only their rates of change are somewhat different. These Lea families are a random sample of the 18 described. If multiple events affect the mRNA abundance of each Lea family, such events then each affect their individual transcripts in a similar way.
在异源四倍体棉花陆地棉（Gossypium hirsutum L. cv Coker 201）胚胎发生过程中，18个棉花Lea（胚胎后期丰富）基因家族呈现出两种相关的总转录本积累模式之一。每个类别中的协同积累很复杂，这表明Lea mRNA丰度受多种事件调控。每个Lea基因家族可能包含两个活跃的同源基因（等位基因），分别位于棉花的两个亚基因组中。一个有趣的问题是，Lea家族的两个转录本是否受到相同的调控，或者总转录本积累的复杂性是否是由于这两个转录本的不同调控所致。7个Lea家族的同源转录本所编码的两种多肽在二维凝胶上可以区分。总RNA中大多数Lea转录本在整个发育过程中在体外都具有功能；因此，体外翻译应该能够准确测量它们的相对丰度。在胚胎成熟期间（Lea转录本浓度平均增加70倍）以及萌发的前12小时（Lea转录本浓度平均下降50倍），跟踪每个家族两个转录本的比例。对于6个Lea家族，在这个时期两个转录本的相对水平保持不变。第七个Lea家族（Lea9）的两个组成转录本在成熟后期相对浓度变化5倍，在萌发期间变化3倍。两个转录本仍然遵循相同的时间积累模式；只是它们的变化速率略有不同。这些Lea家族是所描述的18个家族中的随机样本。如果多个事件影响每个Lea家族的mRNA丰度，那么这些事件对它们各自的转录本的影响方式相似。
