陆地棉晚期胚胎发生丰富（LEA）蛋白家族的特征及其在耐旱性中的作用
Characterization of the late embryogenesis abundant (LEA) proteins family and their role in drought stress tolerance in upland cotton.
作者信息
Magwanga Richard Odongo, Lu Pu, Kirungu Joy Nyangasi, Lu Hejun, Wang Xingxing, Cai Xiaoyan, Zhou Zhongli, Zhang Zhenmei, Salih Haron, Wang Kunbo, Liu Fang
机构信息
Institute of Cotton Research, Chinese Academy of Agricultural Science (ICR, CAAS)/State Key Laboratory of Cotton Biology, Anyang, 455000, China.
School of physical and biological sciences (SPBS), Main campus, Jaramogi Oginga Odinga University of Science and Technology (JOOUST), P.O Box 210-40601, Bondo, Kenya.
出版信息
BMC Genet. 2018 Jan 15;19(1):6. doi: 10.1186/s12863-017-0596-1.
BACKGROUND
Late embryogenesis abundant (LEA) proteins are large groups of hydrophilic proteins with major role in drought and other abiotic stresses tolerance in plants. In-depth study and characterization of LEA protein families have been carried out in other plants, but not in upland cotton. The main aim of this research work was to characterize the late embryogenesis abundant (LEA) protein families and to carry out gene expression analysis to determine their potential role in drought stress tolerance in upland cotton. Increased cotton production in the face of declining precipitation and availability of fresh water for agriculture use is the focus for breeders, cotton being the backbone of textile industries and a cash crop for many countries globally.
RESULTS
In this work, a total of 242, 136 and 142 LEA genes were identified in G. hirsutum, G. arboreum and G. raimondii respectively. The identified genes were classified into eight groups based on their conserved domain and phylogenetic tree analysis. LEA 2 were the most abundant, this could be attributed to their hydrophobic character. Upland cotton LEA genes have fewer introns and are distributed in all chromosomes. Majority of the duplicated LEA genes were segmental. Syntenic analysis showed that greater percentages of LEA genes are conserved. Segmental gene duplication played a key role in the expansion of LEA genes. Sixty three miRNAs were found to target 89 genes, such as miR164, ghr-miR394 among others. Gene ontology analysis revealed that LEA genes are involved in desiccation and defense responses. Almost all the LEA genes in their promoters contained ABRE, MBS, W-Box and TAC-elements, functionally known to be involved in drought stress and other stress responses. Majority of the LEA genes were involved in secretory pathways. Expression profile analysis indicated that most of the LEA genes were highly expressed in drought tolerant cultivars Gossypium tomentosum as opposed to drought susceptible, G. hirsutum. The tolerant genotypes have a greater ability to modulate genes under drought stress than the more susceptible upland cotton cultivars.
CONCLUSION
The finding provides comprehensive information on LEA genes in upland cotton, G. hirsutum and possible function in plants under drought stress.
背景
胚胎后期丰富（LEA）蛋白是一大类亲水性蛋白，在植物耐旱及其他非生物胁迫耐受性中起主要作用。其他植物中已对LEA蛋白家族进行了深入研究和表征，但陆地棉中尚未开展此类研究。本研究工作的主要目的是表征胚胎后期丰富（LEA）蛋白家族，并进行基因表达分析以确定其在陆地棉耐旱胁迫中的潜在作用。面对降水量下降和农业用水供应减少的情况，提高棉花产量是育种者关注的焦点，棉花是纺织工业的支柱，也是全球许多国家的经济作物。
结果
在本研究中，分别在陆地棉、亚洲棉和雷蒙德氏棉中鉴定出242个、136个和142个LEA基因。根据保守结构域和系统发育树分析，将鉴定出的基因分为八组。LEA 2组最为丰富，这可能归因于其疏水性特征。陆地棉LEA基因内含子较少，分布于所有染色体上。大多数重复的LEA基因是片段重复。共线性分析表明，较大比例的LEA基因是保守的。片段基因重复在LEA基因的扩增中起关键作用。发现63个miRNA靶向89个基因，如miR164、ghr-miR394等。基因本体分析表明，LEA基因参与脱水和防御反应。几乎所有LEA基因的启动子都含有ABRE、MBS、W-Box和TAC元件，这些元件在功能上已知参与干旱胁迫和其他胁迫反应。大多数LEA基因参与分泌途径。表达谱分析表明，与干旱敏感的陆地棉相比，大多数LEA基因在耐旱品种海岛棉中高表达。与更敏感的陆地棉品种相比，耐旱基因型在干旱胁迫下调节基因的能力更强。
结论
该研究结果提供了关于陆地棉中LEA基因及其在干旱胁迫下植物中可能功能的全面信息。
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