在对穿透敏感的胚胎中直接显微注射物质。

Direct microinjection of substances in penetration-sensitive embryos.

作者信息

Kühtreiber W M, Serras F

出版信息

Differentiation. 1987;34(2):156-9. doi: 10.1111/j.1432-0436.1987.tb00062.x.

DOI:10.1111/j.1432-0436.1987.tb00062.x

Abstract

We have modified an electrode holder into an oil-pressure holder by fitting an internal platinum heating filament into the holder. This design enables easy preparation, mounting, and exchange of micropipettes. Micropipettes with very thin tips (outer diameter less than 0.1 micron) can be used, as the system allows the use of back-filled pipettes. Therefore, successful microinjections in penetration-sensitive embryos are possible. The characteristics of the system are described, and its advantages and disadvantages, as compared to other microinjection systems, are discussed.

摘要

我们通过在电极架内安装内部铂加热丝，将电极架改装成了油压架。这种设计使得微量移液器的制备、安装和更换都很容易。由于该系统允许使用回充移液器，因此可以使用尖端非常细（外径小于0.1微米）的微量移液器。因此，在对穿透敏感的胚胎中成功进行显微注射是可能的。描述了该系统的特性，并讨论了与其他显微注射系统相比其优缺点。