Samaee Seyed-Mohammadreza, Nikkhah Hanieh, Varga Zoltan M, Rezaei Bahman
1 Aquatic Laboratory, Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Urmia University , Urmia, Iran .
2 Zebrafish International Resource Center, Institute of Neuroscience, University of Oregon , Eugene, Oregon.
Zebrafish. 2017 Dec;14(6):581-585. doi: 10.1089/zeb.2017.1425. Epub 2017 Jul 5.
Microinjection is a widely used technique to inject defined volumes and concentrations of substances and explore their physiological function in vivo. The technique has been particularly successful with zebrafish embryos; however, the injection equipment can be relatively expensive and therefore available only to well-funded laboratories. In this study, a simple, cheap, easy-to-assemble, and easy-to-use setup with a straightforward, accurate, and efficacious calibration method is introduced. The accuracy of this calibration method was tested by comparing with the results of calibration methods that are currently used in high-cost systems. Injection success with this low-cost system was verified based on the presence of injected dyes in zebrafish embryos, the absence of any significant morphological and behavioral differences between 3,4,-dichloroaniline-treated and untreated embryos, and larval viability.
显微注射是一种广泛应用的技术，用于注射特定体积和浓度的物质，并在体内探索其生理功能。该技术在斑马鱼胚胎上尤为成功；然而，注射设备可能相对昂贵，因此只有资金充足的实验室才能使用。在本研究中，介绍了一种简单、廉价、易于组装且易于使用的装置，以及一种直接、准确且有效的校准方法。通过与目前在高成本系统中使用的校准方法的结果进行比较，测试了该校准方法的准确性。基于斑马鱼胚胎中注射染料的存在、3,4-二氯苯胺处理和未处理胚胎之间没有任何明显的形态和行为差异以及幼虫活力，验证了该低成本系统的注射成功率。
