Xin Hui, Deng Chenggang, Zhou Hongmei, Sang Xuehao, Chen Bin
State Key Laboratory of Multiphase Flow in Power Engineering, Xi'an Jiaotong University, Xi'an, Shaanxi, China.
Department of Dermatology, 2nd Affiliated Hospital, Medical School, Laser Treatment Center, Xi'an Jiaotong University, Xi'an, Shaanxi, China.
Lasers Surg Med. 2022 Dec;54(10):1298-1308. doi: 10.1002/lsm.23607. Epub 2022 Oct 13.
Owing to the greater absorption affinity for lipo-rich tissue than water, the 1210 nm laser is a promising candidate for transcutaneous lipolysis in the near-infrared band. However, fat reduction is limited because laser therapy may yield thermal injury of normal tissue. A new protocol to incorporate multipulsed cryogen spray cooling is beneficial to improve the lipolysis effect, and the parameters of laser and cooling can be optimized via skin histopathological analysis.
A murine in vivo model of inguinal tissue of SD rats was established to test the effectivity of transcutaneous lipolysis protocol by R134a multipulsed spray cooling assisted 1210 nm laser irradiation. Tissue response of lipolysis with/without cooling 10 days post the treatment was evaluated by histopathological analysis of skin samples stained with hematoxylin-eosin (HE), through which safe and effective parameters for lipolysis were determined.
From histopathological analysis of the inguinal tissue of SD rats irradiated by the 1210 nm laser alone, the optimal durations are respectively 7 and 3 s (seconds) for low-dosage (6 W) and high-dosage (9 W) therapy, with pronounced lipolysis effect and minimum injury of skin tissue. The multipulsed spray cooling by R134a with a pulse duration of 10 ms (milliseconds), a pulse delay of 2000 ms, and a pulse number of 5 can be introduced to assist the 1210 nm laser therapy with a power of 9 W and a duration of 7 s to achieve desirable fat liquefaction while keeping the complete structure of skin tissue as well as esthetic-related beneficial effects of hair removal and skin rejuvenation.
Excellent lipolysis effect can be achieved via R134a multipulsed spray cooling assisted high-dosage 1210 nm laser irradiation with reasonably matched laser and cooling parameters. The protocol is as follows: Start MP-CSC for one cycle, and then fire the laser with specific power and duration, while keeping MP-CSC accordingly. This new protocol may promote the safe and effective clinical implement of transcutaneous laser lipolysis in body contouring.
由于1210 nm激光对富含脂肪组织的吸收亲和力高于水，因此它是近红外波段经皮脂肪分解的一个有潜力的选择。然而，脂肪减少有限，因为激光治疗可能会对正常组织造成热损伤。一种纳入多脉冲冷冻剂喷雾冷却的新方案有利于提高脂肪分解效果，并且可以通过皮肤组织病理学分析来优化激光和冷却参数。
建立SD大鼠腹股沟组织的小鼠体内模型，以测试R134a多脉冲喷雾冷却辅助1210 nm激光照射的经皮脂肪分解方案的有效性。通过苏木精-伊红（HE）染色的皮肤样本的组织病理学分析，评估治疗后10天有/无冷却的脂肪分解组织反应，由此确定脂肪分解的安全有效参数。
通过对单独接受1210 nm激光照射的SD大鼠腹股沟组织进行组织病理学分析，低剂量（6 W）和高剂量（9 W）治疗的最佳持续时间分别为7秒和3秒，脂肪分解效果显著且皮肤组织损伤最小。可以引入脉冲持续时间为10毫秒、脉冲延迟为200毫秒且脉冲数为5的R134a多脉冲喷雾冷却，以辅助功率为9 W、持续时间为7秒的1210 nm激光治疗，在保持皮肤组织完整结构以及脱毛和皮肤年轻化等美学相关有益效果的同时，实现理想的脂肪液化。
通过R134a多脉冲喷雾冷却辅助高剂量1210 nm激光照射，以及合理匹配的激光和冷却参数，可实现优异的脂肪分解效果。方案如下：启动多脉冲冷冻剂喷雾冷却一个周期，然后以特定功率和持续时间发射激光，同时相应地保持多脉冲冷冻剂喷雾冷却。这种新方案可能会促进经皮激光脂肪分解在身体塑形中的安全有效临床应用。
