Graham M F, Drucker D E, Perr H A, Diegelmann R F, Ehrlich H P
Gastroenterology. 1987 Oct;93(4):801-9. doi: 10.1016/0016-5085(87)90443-4.
The effect of heparin on human intestinal smooth muscle cell proliferation, collagen and noncollagen protein synthesis, and collagen lattice contraction was studied in vitro. Proliferation of serum-stimulated cells was inhibited in a concentration-related fashion by continuous exposure to heparin. The inhibition of proliferation was reversible when heparin was removed from the culture medium. Collagen synthesis was inhibited by 24-h exposure to heparin, but only during that phase of culture (8-12 days) when collagen synthesis was maximal. Noncollagen protein synthesis was down-regulated by 24-h exposure to heparin at all phases of culture tested (5-21 days). Heparin also abolished the contraction of collagen lattices populated by human intestinal smooth muscle cells. These studies demonstrate that heparin plays a significant role in the modulation of human intestinal smooth muscle cell behavior in vitro and suggest that a similar role is played by heparinlike components of the extracellular matrix in vivo.
在体外研究了肝素对人肠道平滑肌细胞增殖、胶原蛋白和非胶原蛋白合成以及胶原晶格收缩的影响。持续暴露于肝素会以浓度相关的方式抑制血清刺激细胞的增殖。当从培养基中去除肝素时，增殖抑制是可逆的。暴露于肝素24小时会抑制胶原蛋白合成，但仅在培养的该阶段（8 - 12天），即胶原蛋白合成最大时。在测试的所有培养阶段（5 - 21天），暴露于肝素24小时会下调非胶原蛋白合成。肝素还消除了由人肠道平滑肌细胞构成的胶原晶格的收缩。这些研究表明，肝素在体外调节人肠道平滑肌细胞行为中起重要作用，并表明细胞外基质中的类肝素成分在体内发挥类似作用。
