School of Health and Kinesiology, University of Nebraska at Omaha, Omaha, NE 68182, USA.
School of Integrated Physiology and Athletic Training, University of Montana, Missoula, MT 59812, USA.
Int J Environ Res Public Health. 2022 Sep 23;19(19):12028. doi: 10.3390/ijerph191912028.
The purpose of this study was to determine the impact of localized cooling of the skeletal muscle during rest on mitochondrial related gene expression. Thermal wraps were applied to the of each limb of 12 participants. One limb received a cold application (randomized) (COLD), while the other did not (RT). Wraps were removed at the 4 h time point and measurements of skin temperature, blood flow, and intramuscular temperature were taken prior to a muscle biopsy. RT-qPCR was used to measure expression of genes associated with mitochondrial development. Skin and muscle temperatures were lower in COLD than RT ( < 0.05). Femoral artery diameter was lower in COLD after 4 h (0.62 ± 0.05 cm, to 0.60 ± 0.05 cm, = 0.018). Blood flow was not different in COLD compared to RT (259 ± 69 mL·min vs. 275 ± 54 mL·min, = 0.20). and expression was higher in COLD relative to RT (1.57-fold, = 0.037 and 1.34-fold, = 0.006, respectively). There was no difference ( > 0.05) in the expression of , , , , , , , , , , or . The impact of this small magnitude of difference in gene expression of and without alterations in other genes are unknown. There appears to be only limited impact of local muscle cooling on the transcriptional response related to mitochondrial development.
本研究旨在确定在休息时对骨骼肌进行局部冷却对与线粒体相关的基因表达的影响。将热裹敷应用于每个肢体的 12 名参与者的 。一条肢体接受冷应用（随机）（COLD），而另一条肢体则不接受（RT）。在 4 小时时间点去除裹敷，并在肌肉活检前测量皮肤温度、血流和肌肉内温度。RT-qPCR 用于测量与线粒体发育相关的基因的表达。COLD 组的皮肤和肌肉温度低于 RT 组（<0.05）。4 小时后 COLD 组股动脉直径较低（0.62 ± 0.05cm 至 0.60 ± 0.05cm，=0.018）。COLD 组与 RT 组的血流无差异（259 ± 69mL·min 与 275 ± 54mL·min，=0.20）。与 RT 相比，COLD 组的 和 表达更高（分别为 1.57 倍，=0.037 和 1.34 倍，=0.006）。其他基因的表达没有差异（>0.05）。 、 、 、 、 、 、 、 、 或 。这种 和 基因表达的微小差异对其他基因没有影响，其影响尚不清楚。局部肌肉冷却对与线粒体发育相关的转录反应似乎只有有限的影响。
Zotero 插件