Institute of Pathology, Laboratory and Forensic Medicine (I-PPerForM), Universiti Teknologi MARA (UiTM), Sungai Buloh Campus, Jalan Hospital, Sungai Buloh 47000, Selangor, Malaysia.
Faculty of Medicine, Universiti Teknologi MARA (UiTM), Sungai Buloh Campus, Jalan Hospital, Sungai Buloh 47000, Selangor, Malaysia.
Int J Environ Res Public Health. 2022 Oct 8;19(19):12878. doi: 10.3390/ijerph191912878.
A systematic review was performed to identify all the related publications describing PCSK9 and atherogenesis biomarkers attenuation associated with a natural product and plant bioactive compounds in in vitro studies. This review emphasized the imprecision and quality of the included research rather than the detailed reporting of the results. Literature searches were conducted in Scopus, PubMed, and Science Direct from 2003 until 2021, following the Cochrane handbook. The screening of titles, abstracts, and full papers was performed by two independent reviewers, followed by data extraction and validity. Study quality and validity were assessed using the Imprecision Tool, Model, and Marker Validity Assessment that has been developed for basic science studies. A total of 403 articles were identified and 31 of those that met the inclusion criteria were selected. 13 different atherogenesis biomarkers in relation to PCSK9 were found, and the most studied biomarkers are LDLR, SREBP, and HNF1α. In terms of quality, our review suggests that the basic science study in investigating atherogenesis biomarkers is deficient in terms of imprecision and validity.
系统评价旨在确定所有相关出版物，描述与体外研究中天然产物和植物生物活性化合物相关的 PCSK9 和动脉粥样硬化生物标志物的衰减。本综述强调了纳入研究的不准确性和质量，而不是详细报告结果。文献检索在 Scopus、PubMed 和 Science Direct 中进行，时间范围为 2003 年至 2021 年，遵循 Cochrane 手册。两名独立评审员对标题、摘要和全文进行筛选，然后进行数据提取和有效性评估。使用为基础科学研究开发的不准确性工具、模型和标志物有效性评估来评估研究质量和有效性。共确定了 403 篇文章，其中 31 篇符合纳入标准。发现了 13 种与 PCSK9 相关的不同动脉粥样硬化生物标志物，研究最多的生物标志物是 LDLR、SREBP 和 HNF1α。就质量而言，我们的综述表明，在调查动脉粥样硬化生物标志物的基础科学研究中，不准确性和有效性存在缺陷。
