Key Laboratory of Forest Genetics & Biotechnology of Ministry of Education of China, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China.
College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, China.
Int J Mol Sci. 2022 Sep 22;23(19):11122. doi: 10.3390/ijms231911122.
is a shrub that can survive in extreme drought environments. The auxin-response factors (ARFs) are a class of transcription factors that are widely involved in plant growth and development, as well as in the regulation of stress resistance. However, the genome-wide identification of the gene family and its responses to environmental stresses, especially drought stress, in has not yet been reported. Here, we identified a total of 12 genes in the genome of , which were distributed over 10 chromosomes and divided into three clades. Intragenome synteny analysis revealed one collinear gene pair in the gene family, i.e., and . Cis-acting element analysis showed that multiple hormones and stress-responsive cis-acting elements were found in the promoters of , suggesting that may be involved in multiple biological processes. Quantitative real-time PCR (qRT-PCR) showed that many had tissue-specific expression patterns, with the highest expression of in the seedlings of . In addition, most of the that were upregulated under drought were independent of endogenous ABA biosynthesis, whereas the response of and to drought was disrupted by the ABA-biosynthesis inhibitor fluridone. These studies provide a basis for further research into how in respond to hormonal signaling and environmental stresses.
是一种能够在极端干旱环境中生存的灌木。生长素响应因子(ARFs)是一类广泛参与植物生长发育以及应激抗性调节的转录因子。然而,关于 基因家族的全基因组鉴定及其对环境胁迫,特别是干旱胁迫的响应,在 中尚未有报道。在这里,我们共鉴定到 基因组中的 12 个 基因,它们分布在 10 条染色体上,并分为 3 个分支。基因组内同线性分析显示, 基因家族中存在一对共线性基因,即 和 。顺式作用元件分析表明,在 的启动子中发现了多个激素和应激响应的顺式作用元件,这表明 可能参与了多个生物学过程。实时定量 PCR(qRT-PCR)显示,许多 具有组织特异性表达模式,其中 基因在幼苗中的表达最高。此外,大多数在干旱条件下上调的 基因不依赖于内源 ABA 生物合成,而 ABA 生物合成抑制剂 fluridone 则破坏了 基因和 基因对干旱的响应。这些研究为进一步研究 如何响应激素信号和环境胁迫提供了基础。