Chongqing Key Laboratory of Microsporidia Infection and Control, Southwest University, Chongqing 400715, China.
State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, China.
Int J Mol Sci. 2022 Sep 30;23(19):11574. doi: 10.3390/ijms231911574.
Enterocytozoon hepatopenaei (EHP) is the pathogen of hepatopancreatic microsporidiosis (HPM) in shrimp. The diseased shrimp Litopenaeus vannamei exhibits a slow growth syndrome, which causes severe economic losses. Herein, 4D label-free quantitative proteomics was employed to analyze the hepatopancreas of L. vannamei with a light (EHPptp2 < 103 copies/50 ng hpDNA, L group) and heavy (EHPptp2 > 104 copies/50 ng hpDNA, H group) load of EHP to better understand the pathogenesis of HPM. Exactly 786 (L group) and 1056 (H group) differentially expressed proteins (DEPs) versus the EHP-free (C group) control were mainly clustered to lipid metabolism, amino acid metabolism, and energy production processing. Compared with the L group, the H group exhibited down-regulation significantly in lipid metabolism, especially in the elongation and degradation of fatty acid, biosynthesis of unsaturated fatty acid, metabolism of α-linolenic acid, sphingolipid, and glycerolipid, as well as juvenile hormone (JH) degradation. Expression pattern analysis showed that the degree of infection was positively correlated with metabolic change. About 479 EHP proteins were detected in infected shrimps, including 95 predicted transporters. These findings suggest that EHP infection induced the consumption of storage lipids and the entire down-regulation of lipid metabolism and the coupling energy production, in addition to the hormone metabolism disorder. These were ultimately responsible for the stunted growth.
对虾微孢子虫病（HPM）的病原体是对虾肠胞内寄生菌（EHP）。受感染的凡纳滨对虾（Litopenaeus vannamei）表现出生长缓慢综合征，这会导致严重的经济损失。在此，采用 4D 无标记定量蛋白质组学分析了轻载（EHPptp2<103 拷贝/50ng hpDNA，L 组）和重载（EHPptp2>104 拷贝/50ng hpDNA，H 组）EHP 对虾肝胰腺，以更好地了解 HPM 的发病机制。与无 EHP（C 组）对照相比，L 组和 H 组分别有 786 个（L 组）和 1056 个（H 组）差异表达蛋白（DEPs），这些蛋白主要聚类为脂质代谢、氨基酸代谢和能量产生过程。与 L 组相比，H 组的脂质代谢显著下调，特别是在脂肪酸的延伸和降解、不饱和脂肪酸的生物合成、α-亚麻酸、鞘脂和甘油脂以及保幼激素（JH）降解方面。表达模式分析表明，感染程度与代谢变化呈正相关。在感染的虾中检测到约 479 种 EHP 蛋白，包括 95 种预测转运蛋白。这些发现表明，EHP 感染诱导了储存脂质的消耗以及整个脂质代谢和偶联能量产生的下调，此外还有激素代谢紊乱。这些最终导致了生长迟缓。
