Department of Psychiatry, University of California San Diego, San Diego, CA 92037, USA.
Department of Entomology and Nematology and Comprehensive Cancer Center, University of California, Davis, CA 95616, USA.
Int J Mol Sci. 2022 Oct 5;23(19):11798. doi: 10.3390/ijms231911798.
The metabolism of bioactive oxylipins by soluble epoxide hydrolase (sEH) plays an important role in inflammation, and sEH may be a risk modifier in various human diseases and disorders. The relationships that sEH has with the risk factors of these diseases remain elusive. Herein, sEH protein expression and activity in white blood cells were characterized before and after a high-fat meal in healthy women (HW) and women with anorexia nervosa (AN). sEH expression and sEH activity were significantly correlated and increased in both groups two hours after consumption of the study meal. Fasting sEH expression and activity were positively associated with body mass index (BMI) in both groups, while an inverse association with age was found in AN only (p value < 0.05). sEH was not associated with anxiety or depression in either group at the fasting timepoint. While the anxiety score decreased after eating in both groups, a higher fasting sEH was associated with a lower postprandial anxiety decrease in HW (p value < 0.05). sEH characterization using direct measurements verified the relationship between the protein expression and in vivo activity of this important oxylipin modulator, while a well-controlled food challenge study design using HW and a clinical control group of women with disordered eating elucidated sEH’s role in the health of adult women.
生物活性氧化脂类通过可溶性环氧化物水解酶（sEH）的代谢在炎症中起着重要作用，sEH 可能是各种人类疾病和障碍的风险修饰因子。sEH 与这些疾病的危险因素之间的关系仍然难以捉摸。在此，研究了健康女性（HW）和神经性厌食症（AN）女性在高脂肪餐后白细胞中 sEH 的蛋白表达和活性的变化。在两组人群中，进食研究餐后两小时 sEH 表达和活性均显著升高且与 sEH 表达呈正相关。两组人群的空腹 sEH 表达和活性均与体重指数（BMI）呈正相关，而 AN 组仅与年龄呈负相关（p 值 < 0.05）。在空腹时，sEH 与两组人群的焦虑或抑郁均无相关性。尽管两组人群在进食后焦虑评分均降低，但 HW 人群中空腹 sEH 水平越高，餐后焦虑评分降低幅度越低（p 值 < 0.05）。使用直接测量方法对 sEH 进行特征描述，验证了该重要氧化脂类调节剂的蛋白表达与其体内活性之间的关系，而使用 HW 作为对照并纳入患有饮食失调的女性临床对照组的严格控制的食物挑战研究设计，则阐明了 sEH 在成年女性健康中的作用。
