State Key Laboratory of Crop Stress Biology in Arid Areas, College of Agronomy, Northwest A & F University, Yangling 712100, China.
Dexing Township Agro-Pastoral Comprehensive Service Center, Nyingchi 860700, China.
Int J Mol Sci. 2022 Oct 5;23(19):11824. doi: 10.3390/ijms231911824.
Foxtail millet () plays an important role in C4 crop research and agricultural development in arid areas due to its short growth period, drought tolerance, and barren tolerance. Exploration of the dwarfing mechanism and the dwarf genes of foxtail millet can provide a reference for dwarf breeding and dwarf research of other C4 crops. In this study, genetic analysis was performed using phenotypic data, candidate genes were screened by bulk segregant analysis sequencing (BSA-Seq); differentially expressed genes and metabolic pathways in different strains of high samples were analyzed by RNA sequencing (RNA-Seq). The association analysis of BSA-Seq and RNA-Seq further narrowed the candidate range. As a result, a total of three quantitative trait loci (QTLs) and nine candidate genes related to plant height were obtained on chromosomes I and IX. Based on the functional prediction of the candidate genes, we propose a hypothetical mechanism for the formation of millet dwarfing, in which, metabolism and MAPK signaling play important roles in the formation of foxtail millet plant height.
谷子在 C4 作物研究和干旱地区农业发展中发挥着重要作用，因为它具有生长周期短、耐旱、耐瘠薄等特点。探索谷子的矮化机制和矮化基因，可以为其他 C4 作物的矮化育种和矮化研究提供参考。本研究利用表型数据进行遗传分析，通过 bulk segregant analysis sequencing (BSA-Seq) 筛选候选基因；通过 RNA 测序 (RNA-Seq) 分析不同高代样本中差异表达基因和代谢途径。BSA-Seq 和 RNA-Seq 的关联分析进一步缩小了候选范围。结果在 I 号和 IX 号染色体上共获得了与株高相关的三个数量性状位点 (QTLs) 和九个候选基因。基于候选基因的功能预测，我们提出了谷子矮化形成的假设机制，其中代谢和 MAPK 信号在谷子株高形成中发挥重要作用。
