Nazish Moona, Althobaiti Ashwaq T
Department of Botany, Rawalpindi Women University, Rawalpindi 46300, Pakistan.
Department of Biology, College of Sciences, Taif University, Ta'if 21974, Saudi Arabia.
Plants (Basel). 2022 Oct 5;11(19):2618. doi: 10.3390/plants11192618.
Pollen morphology of 12 salt-tolerant grasses (, , , , , , , , , , , and ) from the Salt Range was studied using scanning electron microscopy (SEM) and light microscopy (LM). The main aim was the elucidation of taxonomic significance of pollen morphology, which might be useful for taxonomists in the identification of halophytic Poaceae taxa. The described pollen morphology is a good source of taxonomic characteristics that can help in species and genera differentiation. The investigated variations in the pollen micromorphological features and exploration of new palynological traits support and strengthen the systematics of Poaceae. The pollen shape of all studied species was sub-spheroidal, and exine ornamentation includes microechinate-areolate (11 spp.) and microechinate (1 spp.). Two types of pollen apertures were reported, i.e., monoporate (11 spp.) and diporate (). The annulus was found in all species while operculum was observed only in three species. The univariate and multivariate analyses were used to analyze the quantitative data. The highest pollen viability values (%) depicted the adaptability of Poaceae taxa in the salt region. Overall, the pollen characteristics in polar and equatorial view, apertures, annulus, operculum, and surface ornamentation of pollen grains of the family Poaceae are of great taxonomic significance for the species identification.
利用扫描电子显微镜（SEM）和光学显微镜（LM）对盐山地区的12种耐盐禾本科植物（、、、、、、、、、、和）的花粉形态进行了研究。主要目的是阐明花粉形态的分类学意义，这可能有助于分类学家鉴定盐生禾本科类群。所描述的花粉形态是分类学特征的良好来源，有助于区分物种和属。对花粉微观形态特征的研究变化以及对新孢粉学特征的探索支持并加强了禾本科的系统学。所有研究物种的花粉形状均为近球形，外壁纹饰包括微刺状-网纹状（11种）和微刺状（1种）。报道了两种类型的花粉萌发孔，即单孔（11种）和双孔（）。在所有物种中均发现有环带，而仅在三个物种中观察到盖。采用单变量和多变量分析来分析定量数据。最高的花粉活力值（%）表明禾本科类群在盐区的适应性。总体而言，禾本科花粉粒在极面和赤道面的特征、萌发孔、环带、盖以及表面纹饰对物种鉴定具有重要的分类学意义。
