Nazish Moona, Zafar Muhammad, Ahmad Mushtaq, Sultana Shazia, Ullah Riaz, Alqahtani Ali S, Ullah Farhat, Ahmad Shafiq, Ashfaq Shomaila, Ullah Fazal
Department of Plant Sciences, Quaid-i-Azam University, Islamabad, Pakistan.
Medicinal, Aromatic & Poisonous Plants Research Center MAPPRC, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia.
Microsc Res Tech. 2019 Mar;82(3):304-316. doi: 10.1002/jemt.23173. Epub 2019 Jan 5.
The pollen morphology of 11 salt tolerant plant species of family Amaranthaceae from the salt range of Northern Punjab, Pakistan has been studied. The palyno-morphological characters were examined using light and scanning electron microscope. The examined all salt tolerant species have a slight difference in size but have similarity in shape, pore ornamentation, and polarity. The observed morphological characters of pollen grains were pollen symmetry, size, shape, pore ornamentation, pore size, number of pores, exine thickness, polar and equatorial diameter and, P/E ratio. Apolar type of pollens has been observed in all species. Shape of pollens was spheroidal. Exine sculpturing of pollen grains was scabrate (six spp), microechinate (four spp), and microechinate-scabrate (one spp). Different pori numbers were observed in different species. The pantoporate aperturate and sunken pore ornamentation have been reported in all species. A pollen taxonomic key was developed using examined morphological characters for the accurate identification of halophytic taxa. The high fertility and low sterility of pollens confirmed that the selected halophytes are well-established in the salt region. The findings highlight the taxonomic significance of pollen morphology in correct identification and differentiation of salt tolerant plant species.
对来自巴基斯坦旁遮普省北部盐山地区的苋科11种耐盐植物的花粉形态进行了研究。利用光学显微镜和扫描电子显微镜对孢粉形态特征进行了观察。所有被研究的耐盐物种在大小上略有差异,但在形状、孔纹饰和极性方面具有相似性。观察到的花粉粒形态特征包括花粉对称性、大小、形状、孔纹饰、孔径、孔数、外壁厚度、极轴和赤道轴直径以及极轴/赤道轴比。在所有物种中均观察到无极型花粉。花粉形状为球形。花粉粒的外壁纹饰为糙面(6种)、微刺状(4种)和微刺状-糙面(1种)。不同物种观察到不同的孔数。所有物种均报道了全孔型有孔且凹陷的孔纹饰。利用观察到的形态特征编制了花粉分类检索表,以准确鉴定盐生类群。花粉的高育性和低不育性证实了所选盐生植物在盐区生长良好。研究结果突出了花粉形态在耐盐植物物种正确鉴定和区分中的分类学意义。