姜黄提取物对地塞米松处理的小鼠和肌管中肌肉萎缩的抑制作用。

Suppressive Effects of Turmeric Extract on Muscle Atrophy in Dexamethasone-Treated Mice and Myotubes.

机构信息

Health Nutrition, Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.

出版信息

Nutrients. 2022 Sep 25;14(19):3979. doi: 10.3390/nu14193979.

DOI:10.3390/nu14193979

PMID:36235632

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9571062/

Abstract

Sarcopenia is the decline in skeletal muscle mass, strength, and functions, which decreases the quality of life in elderly people. This study investigated the suppressive effect of turmeric (Curcuma longa) extract (TE) on muscle atrophy in dexamethasone (DEX)-treated mice and C2C12 myotubes. DEX treatment significantly decreased the muscle weight and significantly increased Fbxo32 and Murf1 expression in mice, and these changes were suppressed by the supplementation of an AIN-93 based diet with 2% TE. A similar pattern was observed in FBXO32 and MuRF1 protein expression. In C2C12 myotubes, DEX treatment significantly increased FBXO32 and MuRF1 gene and protein expression, and these increases were significantly suppressed by TE supplementation at a concentration of 200 µg/mL. Furthermore, one of the five TE fractions, which were separated by high-performance liquid chromatography had a similar effect with TE supplementation. The present study proposes the suppressive effect of turmeric on sarcopenia.

摘要

肌肉减少症是骨骼肌质量、力量和功能的下降，会降低老年人的生活质量。本研究探讨了姜黄（Curcuma longa）提取物（TE）对地塞米松（DEX）处理的小鼠和 C2C12 肌管萎缩的抑制作用。DEX 处理显著降低了小鼠的肌肉重量，并显著增加了 Fbxo32 和 Murf1 的表达，而在 AIN-93 基础饮食中添加 2%TE 可抑制这些变化。FBXO32 和 MuRF1 蛋白表达也呈现出类似的模式。在 C2C12 肌管中，DEX 处理显著增加了 FBXO32 和 MuRF1 基因和蛋白的表达，而在 200µg/mL 浓度下添加 TE 可显著抑制这些增加。此外，通过高效液相色谱法分离的 TE 的五个部分之一也具有与 TE 补充相似的作用。本研究提出了姜黄对肌肉减少症的抑制作用。