Karpova Svetlana G, Varyan Ivetta A, Olkhov Anatoly A, Popov Anatoly A
Department of Biological and Chemical Physics of Polymers, Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, 4 Kosygina Street, 119334 Moscow, Russia.
Academic Department of Innovational Materials and Technologies Chemistry, Plekhanov Russian University of Economics, 36 Stremyanny lane, 117997 Moscow, Russia.
Polymers (Basel). 2022 Sep 27;14(19):4055. doi: 10.3390/polym14194055.
Comprehensive studies combining X-ray diffraction analysis, thermophysical, dynamic measurements by probe method and scanning electron microscopy have been carried out. The peculiarity of the crystalline and amorphous structure of ultra-thin fibers based on poly(3-hydroxybutyrate) (PHB) containing minor concentrations (0-5%) of a gene and a tetraphenylporphyrin (TFP) complex with iron (in the form of FeCl) are considered. When these complexes are added to the PHB fibers, the morphology of the fibers change: a sharp change in the crystallinity and molecular mobility in the amorphous regions of PHB is observed. When adding a gel to the fibers of PHB, a significant decrease in the degree of crystallinity, melting enthalpy, and correlation time can be observed. The reverse pattern is observed in a system with the addition of FeCl-TFP-there is a significant increase in the degree of crystallinity, melting enthalpy and correlation time. Exposure of PHB fibers with gemin in an aqueous medium at 70 °C leads to a decrease in the enthalpy of melting in modified fibers-to an increase in this parameter. The molecular mobility of chains in amorphous regions of PHB/gemin fibers increases at the same time, a nonlinear dependence of changes in molecular dynamics is observed in PHB/FeCl-TFP fibers. Ozonolysis has a complex effect on the amorphous structure of the studied systems. The obtained fibrous materials have bactericidal properties and should be used in the creation of new therapeutic systems of antibacterial and antitumor action.
已经开展了结合X射线衍射分析、热物理性质、探针法动态测量以及扫描电子显微镜的综合研究。研究了基于聚（3-羟基丁酸酯）（PHB）的超细纤维的晶体和非晶结构的特性，这些纤维含有低浓度（0-5%）的基因以及与铁（以FeCl形式）形成的四苯基卟啉（TFP）配合物。当将这些配合物添加到PHB纤维中时，纤维的形态会发生变化：观察到PHB非晶区域的结晶度和分子流动性发生急剧变化。当向PHB纤维中添加凝胶时，可以观察到结晶度、熔化焓和相关时间显著降低。在添加FeCl-TFP的体系中观察到相反的模式——结晶度、熔化焓和相关时间显著增加。在70°C的水介质中用双子表面活性剂处理PHB纤维会导致改性纤维的熔化焓降低——该参数增加。同时，PHB/双子表面活性剂纤维非晶区域中链的分子流动性增加，在PHB/FeCl-TFP纤维中观察到分子动力学变化的非线性依赖性。臭氧分解对所研究体系的非晶结构有复杂的影响。所获得的纤维材料具有杀菌性能，应被用于创建具有抗菌和抗肿瘤作用的新型治疗体系。
