Stills H F, Hook R R, Sprouse R F
Infect Immun. 1987 Sep;55(9):2240-6. doi: 10.1128/iai.55.9.2240-2246.1987.
The role of Campylobacter jejuni in the pathogenesis of proliferative ileitis in Syrian hamsters was evaluated with monoclonal antibodies of different specificities. Monoclonal antibodies were produced with two different specificities: one for all members of the genus Campylobacter tested (antibody 8322-2E6) and one for C. jejuni and Campylobacter coli (antibodies 841-2A11, 841-4C6, and 841-5B1). Heal sections from healthy hamsters, from hamsters with naturally occurring proliferative ileitis, and from hamsters with experimentally induced proliferative ileitis were examined by using fluorescein isothiocyanate-labeled, Campylobacter sp.-specific 8322-2E6 and tetramethylrhodamine isothiocyanate-labeled C. jejuni-C. coli-specific 841-2A11 for direct dual-labeling immunofluorescence. Organisms which stained with the C. jejuni-C. coli-specific monoclonal antibody were observed in the ileal lumens and along the distal tips of the villi of hamsters with either experimentally induced or naturally occurring proliferative ileitis. In contrast, organisms identified by the Campylobacter sp.-specific monoclonal antibody were present deep within the villus lumens and crypts and intracellularly within the apical portions of the epithelial cells. No organisms stained with the C. jejuni-C. coli-specific monoclonal antibody were observed in ileal sections from control hamsters; an occasional intracellular organism stained with the Campylobacter sp.-specific monoclonal antibody was observed in 2 of 10 control hamsters. Thus, at least two immunologically distinct patterns were identified in ileal sections from hamsters with proliferative ileitis. On the basis of these results, we conclude that the organism seen intracellularly in ileal sections from hamsters with proliferative ileitis is a member of the genus Campylobacter but that it probably is not C. jejuni or C. coli.
利用不同特异性的单克隆抗体评估了空肠弯曲菌在叙利亚仓鼠增生性回肠炎发病机制中的作用。制备了具有两种不同特异性的单克隆抗体：一种针对所检测的弯曲菌属所有成员（抗体8322 - 2E6），另一种针对空肠弯曲菌和结肠弯曲菌（抗体841 - 2A11、841 - 4C6和841 - 5B1）。使用异硫氰酸荧光素标记的弯曲菌属特异性8322 - 2E6和异硫氰酸四甲基罗丹明标记的空肠弯曲菌 - 结肠弯曲菌特异性841 - 2A11，对来自健康仓鼠、患有自然发生的增生性回肠炎的仓鼠以及实验性诱导增生性回肠炎的仓鼠的回肠切片进行直接双标记免疫荧光检查。在用空肠弯曲菌 - 结肠弯曲菌特异性单克隆抗体染色的生物样本中，在实验性诱导或自然发生增生性回肠炎的仓鼠的回肠腔以及绒毛远端尖端观察到了这些生物。相比之下，通过弯曲菌属特异性单克隆抗体鉴定出的生物存在于绒毛腔和隐窝深处以及上皮细胞顶端部分的细胞内。在对照仓鼠的回肠切片中未观察到用空肠弯曲菌 - 结肠弯曲菌特异性单克隆抗体染色的生物；在10只对照仓鼠中有2只偶尔观察到用弯曲菌属特异性单克隆抗体染色的细胞内生物。因此，在患有增生性回肠炎的仓鼠的回肠切片中至少鉴定出了两种免疫上不同的模式。基于这些结果，我们得出结论，在患有增生性回肠炎的仓鼠的回肠切片中细胞内所见的生物是弯曲菌属的成员，但它可能不是空肠弯曲菌或结肠弯曲菌。
