Monfort J D, Bech-Nielsen S, Stills H F
Department of Veterinary Preventive Medicine, Ohio State University College of Veterinary Medicine, Columbus 43210.
Vet Res Commun. 1994;18(2):85-92. doi: 10.1007/BF01839224.
A new diagnostic procedure was developed to detect the flagellar antigen of Campylobacter jejuni and Campylobacter coli in canine faecal specimens and was tested on faecal samples from random-source dogs obtained from the local dog pound. Extraction of acid-soluble proteins was performed on faecal specimens and the extracted material was evaluated using species-specific monoclonal antibodies in an enzyme-linked immunosorbent assay. The assay detected all C. jejuni or C. coli infected specimens compared with direct selective faecal culture. One of 18 faecal specimens culture-negative for C. jejuni was identified as positive by the assay, i.e. a false positive rate of 1 of 18 (5.6%) and a corresponding specificity of 94.4%. These results suggest that the screening procedure developed to detect flagellar antigens of C. jejuni and C. coli in canine faecal samples should be further investigated as a diagnostic alternative to culture.
一种新的诊断方法被开发出来，用于检测犬粪便样本中结肠弯曲杆菌和空肠弯曲杆菌的鞭毛抗原，并在从当地动物收容所获取的随机来源犬的粪便样本上进行了测试。对粪便样本进行酸溶性蛋白提取，并在酶联免疫吸附试验中使用种特异性单克隆抗体对提取的物质进行评估。与直接选择性粪便培养相比，该检测方法检测出了所有感染空肠弯曲杆菌或结肠弯曲杆菌的样本。18份空肠弯曲杆菌粪便培养阴性样本中有1份被该检测方法鉴定为阳性，即假阳性率为1/18（5.6%），相应的特异性为94.4%。这些结果表明，为检测犬粪便样本中空肠弯曲杆菌和结肠弯曲杆菌鞭毛抗原而开发的筛查程序，作为一种替代培养的诊断方法，应进一步研究。
