担子菌菌盖、菌丝体和孢子提取物的抗原性/变应原性分析。

Antigenic/allergenic analysis of basidiomycete cap, mycelia, and spore extracts.

作者信息

Weissman D N, Halmepuro L, Salvaggio J E, Lehrer S B

出版信息

Int Arch Allergy Appl Immunol. 1987;84(1):56-61. doi: 10.1159/000234398.

Abstract

Since airborne basidiospores may be important inducers of respiratory allergy, extracts of spores, caps and mycelia from Pleurotus ostreatus were studied by immunologic techniques. Crossed immunoelectrophoresis of the Pleurotus spore extract showed it to be a complex mixture containing at least 27 precipitating antigens. Crossed-line immunoelectrophoresis comparing Pleurotus spore extract with extracts of Pleurotus cap or mycelia demonstrated both common antigens and antigens unique to the spore extract. Inhibition of Pleurotus spore RAST by extracts of Pleurotus spore, cap, and mycelia suggested that these extracts contained common allergenic components. However, wheal and flare skin reactivity of allergic patients to extracts of P. ostreatus or Cantharellus cibarius demonstrated little correlation between reactivity to cap and spores. These results demonstrate that basidiomycete spore extracts are the best diagnostic reagents to use in clinical studies, although cap and mycelia extracts may provide useful material for further allergen analysis.

摘要

由于气传担子孢子可能是呼吸道过敏的重要诱发因素，因此采用免疫技术对平菇的孢子、菌盖和菌丝体提取物进行了研究。平菇孢子提取物的交叉免疫电泳显示，它是一种复杂的混合物，至少含有27种沉淀抗原。将平菇孢子提取物与平菇菌盖或菌丝体提取物进行交叉线免疫电泳，结果表明既有共同抗原，也有孢子提取物特有的抗原。平菇孢子、菌盖和菌丝体提取物对平菇孢子RAST的抑制作用表明，这些提取物含有共同的变应原成分。然而，过敏患者对平菇或鸡油菌提取物的风团和潮红皮肤反应表明，对菌盖和孢子的反应性之间几乎没有相关性。这些结果表明，担子菌孢子提取物是临床研究中最佳的诊断试剂，尽管菌盖和菌丝体提取物可能为进一步的变应原分析提供有用的材料。