Hunan Engineering Research Center of Agricultural Pest Early Warning and Control, Hunan Agricultural University, Changsha City, China.
Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Changsha City, China.
Front Cell Infect Microbiol. 2022 Sep 27;12:980970. doi: 10.3389/fcimb.2022.980970. eCollection 2022.
is a pathogenic fungus that causes serious leaf or flower blight on some medicinal plants worldwide. In this study, multiple dsRNA bands in the range of 1.2-10 kbp were found in a strain HNSZ-1, and eleven full-length cDNA sequences of these dsRNA were obtained by high-throughput sequencing, RT-PCR detection and conventional Sanger sequencing. Homology search and phylogenetic analyses indicated that the strain HNSZ-1 was infected by at least nine mycoviruses. Among the nine, five viruses were confirmed to represent novel viruses in the families , , and a provisional family Ambiguiviridae. Virus elimination and horizontal transmission indicated that the (-) ssRNA virus, AdNSRV1, might be associated with the slow growth and irregular colony phenotype of the host fungus. As far as we know, this is the first report for virome characterization of , which might provide important insights for screening of mycovirus for biological control and for studying of the interactions between viruses or viruses and their host.
是一种致病真菌，可导致全球一些药用植物的严重叶片或花朵枯萎病。在本研究中，在菌株 HNSZ-1 中发现了多个大小在 1.2-10 kbp 之间的 dsRNA 条带，并通过高通量测序、RT-PCR 检测和常规 Sanger 测序获得了这 11 个 dsRNA 的全长 cDNA 序列。同源性搜索和系统发育分析表明，菌株 HNSZ-1 感染了至少 9 种真菌病毒。在这 9 种病毒中，有 5 种病毒被证实代表了科、科、科和暂定科 Ambiguiviridae 的新型病毒。病毒消除和水平传播表明，(-)ssRNA 病毒 AdNSRV1 可能与宿主真菌的生长缓慢和不规则菌落表型有关。据我们所知，这是对的病毒组特征的首次报道，这可能为筛选生物防治中的真菌病毒以及研究病毒或病毒与其宿主之间的相互作用提供重要的见解。
