Li Bo, Cao Yuhan, Ji Zixuan, Zhang Jingyi, Meng Xianglong, Dai Pengbo, Hu Tongle, Wang Shutong, Cao Keqiang, Wang Yanan
State Key Laboratory of North China Crop Improvement and Regulation, College of Plant Protection, Hebei Agricultural University, Baoding, China.
Front Microbiol. 2022 Jun 9;13:910712. doi: 10.3389/fmicb.2022.910712. eCollection 2022.
leaf blotch caused by apple pathotype () is an important fungal disease that affects the production of apples worldwide. Mycoviruses harbored in plant pathogenic fungi can confer hypovirulence in their hosts and have attracted widespread attention as potential biocontrol tools. In this study, the coinfection of two mycoviruses, named chrysovirus 1 strain QY2 (AaCV1-QY2) and magoulivirus 1 (AaMV1), respectively, were isolated from f. sp. strain QY21. Sequence analyses revealed that AaCV1-QY2 virus belonged to the genus and AaMV1 virus belonged to the genus These two mycoviruses were found to be associated with hypovirulence in , among which AaCV1-QY2 might play a relatively leading role. Because the elimination of AaMV1 from the strain QY21 does not affect the hypovirulence trait, which indicates that the virus AaCV1-QY2 can independently induce slow growth and reduce host virulence. Moreover, the presence of viruses decreased the accumulation of the mycotoxin alternariol (AOH) in strains. Intriguingly, AaCV1-QY2/AaMV1 mycoviruses can be horizontally transmitted to other strains, and this coinfection can promote the interspecific transmission efficiency of AaCV1-QY2. To our knowledge, this study reports the first description of the member of is related to hypovirulence in spp. that facilitates the development of biocontrol measures of Roberts.
由苹果致病型（）引起的叶斑病是一种影响全球苹果生产的重要真菌病害。植物病原真菌中携带的真菌病毒可使其宿主表现出低毒力，作为潜在的生物防治工具已引起广泛关注。在本研究中，分别从f. sp. 菌株QY21中分离出两种真菌病毒的共感染，命名为金黄病毒1株QY2（AaCV1-QY2）和马古病毒1（AaMV1）。序列分析表明，AaCV1-QY2病毒属于属，AaMV1病毒属于属。发现这两种真菌病毒与的低毒力有关，其中AaCV1-QY2可能起相对主导作用。因为从菌株QY21中消除AaMV1并不影响低毒力性状，这表明病毒AaCV1-QY2可独立诱导生长缓慢并降低宿主毒力。此外，病毒的存在降低了菌株中霉菌毒素交链孢酚（AOH）的积累。有趣的是，AaCV1-QY2/AaMV1真菌病毒可水平传播到其他菌株，这种共感染可提高AaCV1-QY2的种间传播效率。据我们所知，本研究首次报道了与spp. 低毒力相关的成员描述，这有助于开发罗伯茨氏菌的生物防治措施。
