Department of Agricultural, Food, Environmental and Animal Sciences, University of Udine, Udine, Italy.
J Anim Physiol Anim Nutr (Berl). 2023 May;107(3):747-753. doi: 10.1111/jpn.13780. Epub 2022 Oct 14.
A new rumen batch fermentation system that allows continuous measures of total gas (GP) and methane production (MP) was tested. The fermentation system is composed of glass bottles connected to gas counters (Ritter Apparatebau GmbH & Co. KG) and an infrared gas analyser that measures the methane concentration. The system allows direct and continuous measurement of GP and MP for accurate kinetic studies. The aim of the work was to test the rumen fermentation system and compare the GP and MP kinetics obtained. Barley meal (BM), alfalfa hay (AH), corn silage (CS), and soya bean hulls (SH) were used as substrates in four consecutive fermentation runs. Cumulative volumes of GP and MP and the percentage of methane on total GP were recorded continuously until 48 h and average values at 1 h intervals were fitted with an exponential model with a lag phase reaching a good fit (R > 0.992). GP and MP reached the highest plateau levels for SH (1836 and 370 ml, respectively; p < 0.01) and the lowest for AH (1000 and 233 ml, respectively). The remaining substrates showed intermediate values. MP kinetics showed a discrete lag phase (from 0.09 to 1.12 h), whereas it was equal to zero for the total GP (except for SH). The methane concentration in gas flowing increased rapidly at the beginning of fermentation (from 0.35 to 0.95 h ) and reached a plateau after approximately 8-12 h. In conclusion, the rumen fermentation system evaluated generates methane data comparable to those reported in the literature and allows simple continuous measurement of methane release throughout fermentation.
一种新的瘤胃批次发酵系统，可连续测量总气体（GP）和甲烷产量（MP），已进行测试。该发酵系统由与气体计数器（Ritter Apparatebau GmbH & Co. KG）和测量甲烷浓度的红外气体分析仪相连的玻璃瓶组成。该系统可直接和连续测量 GP 和 MP，从而进行准确的动力学研究。该研究的目的是测试瘤胃发酵系统并比较获得的 GP 和 MP 动力学。大麦粉（BM）、苜蓿干草（AH）、玉米青贮（CS）和大豆皮（SH）分别用作四个连续发酵运行的底物。连续记录 GP 和 MP 的累积体积和甲烷占总 GP 的百分比，直到 48 小时，并以指数模型拟合 1 小时间隔的平均值，具有良好拟合的滞后期（R > 0.992）。SH 的 GP 和 MP 达到最高平台水平（分别为 1836 和 370ml，p<0.01），AH 的最低（分别为 1000 和 233ml）。其余底物显示出中间值。MP 动力学表现出离散的滞后期（从 0.09 到 1.12 小时），而对于总 GP 则为零（SH 除外）。发酵开始时，流动气体中的甲烷浓度迅速增加（从 0.35 到 0.95 小时），大约 8-12 小时后达到平台。总之，所评估的瘤胃发酵系统生成的甲烷数据与文献中报道的数据相当，并且允许在整个发酵过程中简单地连续测量甲烷释放。
