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气体奋进号:一种用于估算瘤胃发酵过程中甲烷动力学的创新设备。

Gas Endeavour: An Innovative Equipment for Estimating Methane Kinetics During Rumen Fermentation.

作者信息

Iqbal Rashid, Arango Sheyla, Tagliapietra Franco, Bailoni Lucia

机构信息

Department of Comparative Biomedicine and Food Science (BCA), University of Padova, 35020 Legnaro, PD, Italy.

Department of Agronomy, Food, Natural Resources, Animals and Environment, University of Padova, 35020 Legnaro, PD, Italy.

出版信息

Animals (Basel). 2025 May 5;15(9):1331. doi: 10.3390/ani15091331.

Abstract

The growing need to reduce methane emissions from ruminants while enhancing feed utilization has driven the development of innovative measurement techniques. This review examines the Gas Endeavour (GES), an automated volumetric apparatus that quantifies both total gas and methane production in real time during rumen fermentation. Utilizing the principles of liquid displacement and buoyancy, the GES integrates a thermostatically controlled water bath, specialized gas flow cells, and an alkaline CO absorption unit to deliver precise kinetic data on fermentation. Compared to conventional methods-which often rely on manual measurements and post-incubation gas chromatography-the GES provides continuous monitoring and immediate data acquisition, reducing labour and potential errors. This review discusses the system's design, operational challenges such as controlling headspace pressure and ensuring consistent inoculum preparation, and its applications in both animal nutrition and biomethane potential assessments. The findings suggest that, with further standardization and protocol refinement, the GES could significantly advance research aimed at optimizing feed digestibility and mitigating methane emissions in ruminant production systems.

摘要

在提高饲料利用率的同时减少反刍动物甲烷排放的需求日益增长,这推动了创新测量技术的发展。本综述考察了气体奋进仪(GES),这是一种自动容积测量装置,可在瘤胃发酵过程中实时量化总气体和甲烷产量。GES利用液体置换和浮力原理,集成了恒温控制水浴、专门的气体流通池和碱性CO吸收单元,以提供关于发酵的精确动力学数据。与通常依赖手动测量和培养后气相色谱分析的传统方法相比,GES可进行连续监测并即时获取数据,减少了劳动力和潜在误差。本综述讨论了该系统的设计、诸如控制顶空压力和确保接种物制备一致性等操作挑战,以及其在动物营养和生物甲烷潜力评估中的应用。研究结果表明,随着进一步的标准化和方案完善,GES能够显著推动旨在优化反刍动物生产系统中饲料消化率和减少甲烷排放的研究。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fad6/12071131/bf8cb44f32ee/animals-15-01331-g001.jpg

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