School of Chemistry and Chemical Engineering, Nanchang University, Nanchang 330031, China.
Institute of Translational Medicine, Nanchang University, Nanchang 330031, China.
ACS Appl Bio Mater. 2022 Nov 21;5(11):5165-5173. doi: 10.1021/acsabm.2c00541. Epub 2022 Oct 14.
Detection and imaging of highly reactive oxygen species (hROS) in biological systems using fluorescent probes are critical for the study of physiological and pathological processes induced by hROS. Herein, we report a redox-active luminescent metal-organic framework (MOF), which incorporates a hydroquinone moiety that can undergo a reversible transformation from the hydroquinone to the quinone by hROS like •OH and ClO. Moreover, the intrinsic fluorescence originating from the excited-state intramolecular proton transfer (ESIPT) property of the organic linker can be finely regulated during this redox-switchable process. A reversible fluorescent probe for hROS is thus developed. The presented probe shows a sensitive, selective, and reversible response to hROS due to the integration of excellent structural characteristics and unique spectral properties of the MOF. The detection limits of •OH and ClO are 0.22 and 0.18 μM, respectively. Furthermore, with good photostability and super biocompatibility, this simple yet efficient fluorescent probe has been successfully applied to dynamic monitoring of endogenous and exogenous •OH and ClO in live cells.
利用荧光探针在生物系统中检测和成像高活性氧（hROS）对于研究 hROS 诱导的生理和病理过程至关重要。在此，我们报告了一种氧化还原活性发光金属-有机骨架（MOF），其中包含一个对苯二酚部分，可通过 hROS 如 •OH 和 ClO 经历从对苯二酚到醌的可逆转化。此外，来自有机配体的激发态分子内质子转移（ESIPT）性质的本征荧光可以在这个氧化还原可切换过程中精细调节。因此，开发了一种用于 hROS 的可逆荧光探针。由于 MOF 的优异结构特征和独特光谱性质的结合，该探针对 hROS 表现出灵敏、选择性和可逆的响应。•OH 和 ClO 的检测限分别为 0.22 和 0.18 μM。此外，该简单而有效的荧光探针具有良好的光稳定性和超生物相容性，已成功应用于活细胞内内源性和外源性 •OH 和 ClO 的动态监测。
