基于金纳米簇@纳米级金属有机骨架的细胞内高活性氧物种的灵敏监测和生物成像。
Sensitive monitoring and bioimaging intracellular highly reactive oxygen species based on gold nanoclusters@nanoscale metal-organic frameworks.
机构信息
Shanghai Key Laboratory of Green Chemistry and Chemical Processes, Department of Chemistry, School of Chemistry and Molecular Engineering, East China Normal University, Shanghai, 200241, China.
Institute for Sustainable Energy/College of Sciences, Shanghai University, Shanghai, 200444, China; Department of Chemical Engineering, Shanghai Jiao Tong University, Shanghai Electrochemical Energy Device Engineering Technology Research Center, Shanghai, 200240, China.
出版信息
Anal Chim Acta. 2019 Dec 27;1092:108-116. doi: 10.1016/j.aca.2019.09.051. Epub 2019 Sep 20.
Nanoscale metal-organic frameworks (NMOFs) have been widely employed in biological diagnosis and treatment system. In this work, glutathione stabilized gold nanoclusters (GSH-AuNCs) were successfully encapsulated in NMOFs and the as-obtained nanocomposites (named as AuNCs@NMOFs) showed nearly 10 times enhancement in fluorescence intensity and longer fluorescence lifetime relative to GSH-AuNCs. In addition, the fluorescence intensity of AuNCs@NMOFs was quenched by highly reactive oxygen species (hROS) due to their strong oxidation ability. Based on the fact, the nanocomposites were used for the detection of hROS with a linear range from 80 nM to 1.0 μM toward hypochlorite (as the representative of hROS). The limit of detection (LOD) was about 30 nM, which was about 30 times lower than that of GSH-AuNCs (LOD = 0.96 μM). With the features of good biocompatibility, excellent luminescence and efficient cellular uptake, AuNCs@NMOFs were successfully applied in fluorescence imaging of hROS in living cells.
纳米尺度金属-有机骨架（NMOFs）已被广泛应用于生物诊断和治疗系统。在这项工作中，谷胱甘肽稳定的金纳米簇（GSH-AuNCs）被成功封装在 NMOFs 中，所得到的纳米复合材料（命名为 AuNCs@NMOFs）表现出比 GSH-AuNCs 强近 10 倍的荧光强度和更长的荧光寿命。此外，由于其强氧化能力，AuNCs@NMOFs 的荧光强度被高活性氧物种（hROS）猝灭。基于这一事实，该纳米复合材料被用于检测 hROS，其线性范围为 80 nM 至 1.0 μM，针对次氯酸盐（作为 hROS 的代表）。检测限（LOD）约为 30 nM，比 GSH-AuNCs 的检测限（LOD=0.96 μM）低约 30 倍。由于具有良好的生物相容性、优异的发光性能和高效的细胞摄取能力，AuNCs@NMOFs 成功地应用于活细胞中 hROS 的荧光成像。
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