Department of Molecular Cell Mechanisms, Medical University of Lodz, Mazowiecka 6/8, 92-215 Lodz, Poland.
Department of Biochemistry, Medical University of Lodz, Mazowiecka 6/8, 92-215 Lodz, Poland.
Food Funct. 2022 Oct 31;13(21):10994-11007. doi: 10.1039/d2fo01737g.
Natural polyphenols are plant metabolites exhibiting a broad range of biological activities. Among them, anticancer properties seem to be very desirable. This study examined the anticancer and anti-metastatic properties of the polyphenol-rich extract from the evening primrose seeds (EPE). and studies performed in colorectal cancer (CRC) cell lines and AOM-DSS-induced colitis-associated colon cancer in mice revealed the EPE anticancer properties. Furthermore, we studied the EPE activity on metastatic abilities and showed that the EPE inhibited invasiveness in the following models (cells isolated from patients with different invasive stages and cells with induced invasion by either Snail overexpression or CAF stimulation). More importantly, we also demonstrated that the EPE decreases the cell invasiveness of 5-fluorouracil (5-FU) resistant CRC cells. The inhibition of metastasis correlated with a decrease in thymidylate synthetase (TYMS), which has recently been associated with metastatic phenotype development. Our results indicate that the EPE might be an effective anticancer agent in suppressing colon cancer metastasis regardless of the invasiveness cause. Based on these findings, we concluded that the used EPE extract rich in polyphenols inhibits cell invasion by TYMS downregulation.
天然多酚是具有广泛生物活性的植物代谢物。其中，抗癌特性似乎非常理想。本研究检查了富含月见草种子（EPE）的多酚提取物的抗癌和抗转移特性。在结直肠癌细胞系和 AOM-DSS 诱导的结肠炎相关结肠癌小鼠中进行的研究揭示了 EPE 的抗癌特性。此外，我们研究了 EPE 对转移能力的活性，并表明 EPE 抑制了以下模型中的侵袭性（从不同侵袭阶段的患者中分离的细胞和通过 SNAIL 过表达或 CAF 刺激诱导侵袭的细胞）。更重要的是，我们还证明 EPE 降低了氟尿嘧啶（5-FU）耐药 CRC 细胞的细胞侵袭性。转移的抑制与胸苷酸合成酶（TYMS）的减少相关，最近与转移表型的发展有关。我们的研究结果表明，EPE 可能是一种有效的抗癌剂，可抑制结肠癌转移，而与侵袭性原因无关。基于这些发现，我们得出结论，使用富含多酚的 EPE 提取物通过下调 TYMS 抑制细胞侵袭。
