School of Water Conservancy and Environment, University of Jinan, Jinan, 250022, PR China.
Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, PR China.
J Environ Manage. 2023 Jan 1;325(Pt A):116430. doi: 10.1016/j.jenvman.2022.116430. Epub 2022 Oct 11.
Although the coupling process of microbial fuel cell (MFC) and activated sludge is widely used for organic matter removal and electric energy recovery, the problem of high effluent nitrate still exists due to the lack of influent carbon source. Herein, a poly (butanediol succinate) (PBS) assembled MFC was established in an aerobic granular sludge (AGS) bioreactor for simultaneous promoting nitrogen removal and electricity generation. Compared to AGS-Control group, the total inorganic nitrogen (TIN) and COD removal efficiencies of AGS-MFC group were improved to 84.3 ± 2.6% and 93.5 ± 0.5% after 100-days operation. The average output voltage and the maximum power density of the MFC module were 223.7 mV and 59.6 mW/m, respectively. Through high-throughput sequencing analysis, Thauera-related denitrifying bacteria had the highest relative abundances (20.0% and 31.4%) in both bioreactors. The relative abundance of Nitrosomonas-related ammonia oxidizing bacteria (AOB) in AGS-MFC (1.8%) was enriched than AGS-Control (1.1%). In MFC module, Thauera (16.2%) with denitrification and power generation was dominant in anodic biofilms under PBS enhancement. This study provides scientific basis for the application of submersible MFC enhanced deep nitrogen removal under aerobic conditions.
虽然微生物燃料电池 (MFC) 与活性污泥的偶联过程广泛用于去除有机物和回收电能，但由于缺乏进水碳源，仍然存在出水硝酸盐含量高的问题。在此，在好氧颗粒污泥 (AGS) 生物反应器中构建了一种聚丁二酸丁二醇酯 (PBS) 组装的 MFC，用于同时促进脱氮和发电。与 AGS-对照相比，AGS-MFC 组在 100 天的运行后，总无机氮 (TIN) 和 COD 的去除效率分别提高到 84.3 ± 2.6%和 93.5 ± 0.5%。MFC 模块的平均输出电压和最大功率密度分别为 223.7 mV 和 59.6 mW/m。通过高通量测序分析，两种生物反应器中都以 Thauera 相关反硝化菌的相对丰度最高（分别为 20.0%和 31.4%）。AGS-MFC 中与亚硝化单胞菌相关的氨氧化菌 (AOB) 的相对丰度（1.8%）比 AGS-对照（1.1%）富集。在 MFC 模块中，在 PBS 增强作用下，具有反硝化和发电功能的 Thauera（16.2%）在阳极生物膜中占主导地位。本研究为潜水式 MFC 在好氧条件下强化深度脱氮的应用提供了科学依据。
