Yang A H, Oberley T D, Oberley L W, Schmid S M, Cummings K B
In Vitro Cell Dev Biol. 1987 Aug;23(8):546-58. doi: 10.1007/BF02620972.
The activities of three antioxidant enzymes, superoxide dismutase, catalase, and glutathione peroxidase, were monitored in isolated human renal adenocarcinoma tissues and in cultured human renal adenocarcinoma cells. The results were compared to the activities of these enzymes in the proposed cell of origin, isolated human proximal tubular tissues, and cultured proximal tubular epithelial cells. Strong modulation of these enzymes by culture conditions was observed in normal cells but not in carcinoma cells. Low levels of cellular lipid peroxidation, as assessed by levels of malondialdehyde (MDA), were observed in adenocarcinoma cells under the culture conditions tested with one exception: greatly elevated MDA was observed in renal adenocarcinoma cells grown on plastic in serum-free, chemically defined medium. This increased lipid peroxidation correlated with a loss of cell viability under these conditions.
在分离出的人肾腺癌组织和培养的人肾腺癌细胞中监测了三种抗氧化酶，即超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶的活性。将结果与这些酶在所提出的起源细胞、分离出的人近端肾小管组织和培养的近端肾小管上皮细胞中的活性进行了比较。在正常细胞中观察到这些酶受培养条件的强烈调节，但在癌细胞中未观察到。通过丙二醛（MDA）水平评估，在测试的培养条件下，腺癌细胞中细胞脂质过氧化水平较低，但有一个例外：在无血清、化学成分确定的培养基中在塑料上生长的肾腺癌细胞中观察到MDA大幅升高。在这些条件下，这种脂质过氧化增加与细胞活力丧失相关。
