Oberley T D, Schultz J L, Oberley L W
Department of Pathology, University of Wisconsin Medical School, Madison.
Free Radic Biol Med. 1994 Jun;16(6):741-51. doi: 10.1016/0891-5849(94)90189-9.
Antioxidant enzyme (AE) activities were studied in normal hamster kidney proximal tubules and in estrogen-induced hamster kidney cancer. In vivo, kidney tumor had lower activities of manganese superoxide dismutase (MnSOD), copper, zinc superoxide dismutase, catalase, and glutathione peroxidase than kidney proximal tubules. Differences in AE activities were, in general, maintained in tissue culture, with AE activities remaining low in tumor cells compared to normal cells. Normal proximal tubular cells showed significant induction of MnSOD activity as a function of time in culture or following exposure to diethylstilbestrol, a synthetic estrogen, while MnSOD activity remained low in tumor cells under these conditions. Our results suggest that antioxidant enzymes, particularly MnSOD, are regulated differently in estrogen-induced hamster kidney tumor cells than in normal kidney proximal tubular cells, demonstrating that cancers arising from hormonal influence have similar AE profiles to those previously described in cancers arising from viral or chemical etiologies.
在正常仓鼠肾近端小管和雌激素诱导的仓鼠肾癌中研究了抗氧化酶（AE）活性。在体内，肾肿瘤中锰超氧化物歧化酶（MnSOD）、铜锌超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶的活性低于肾近端小管。一般来说，AE活性的差异在组织培养中得以维持，与正常细胞相比，肿瘤细胞中的AE活性仍然较低。正常近端小管细胞在培养过程中或暴露于合成雌激素己烯雌酚后，MnSOD活性随时间有显著诱导，而在这些条件下肿瘤细胞中的MnSOD活性仍然较低。我们的结果表明，抗氧化酶，尤其是MnSOD，在雌激素诱导的仓鼠肾肿瘤细胞中的调节方式与正常肾近端小管细胞不同，这表明激素影响引发的癌症具有与先前描述的由病毒或化学病因引起的癌症相似的AE谱。
